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Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines

The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of Trianthema portulacastr...

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Autores principales: Sarfraz, Muhammad Hassan, Zubair, Muhammad, Aslam, Bilal, Ashraf, Asma, Siddique, Muhammad Hussnain, Hayat, Sumreen, Cruz, Jorrdy Neves, Muzammil, Saima, Khurshid, Mohsin, Sarfraz, Muhammad Farrukh, Hashem, Abeer, Dawoud, Turki M., Avila-Quezada, Graciela Dolores, Abd_Allah, Elsayed Fathi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264586/
https://www.ncbi.nlm.nih.gov/pubmed/37323910
http://dx.doi.org/10.3389/fmicb.2023.1188743
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author Sarfraz, Muhammad Hassan
Zubair, Muhammad
Aslam, Bilal
Ashraf, Asma
Siddique, Muhammad Hussnain
Hayat, Sumreen
Cruz, Jorrdy Neves
Muzammil, Saima
Khurshid, Mohsin
Sarfraz, Muhammad Farrukh
Hashem, Abeer
Dawoud, Turki M.
Avila-Quezada, Graciela Dolores
Abd_Allah, Elsayed Fathi
author_facet Sarfraz, Muhammad Hassan
Zubair, Muhammad
Aslam, Bilal
Ashraf, Asma
Siddique, Muhammad Hussnain
Hayat, Sumreen
Cruz, Jorrdy Neves
Muzammil, Saima
Khurshid, Mohsin
Sarfraz, Muhammad Farrukh
Hashem, Abeer
Dawoud, Turki M.
Avila-Quezada, Graciela Dolores
Abd_Allah, Elsayed Fathi
author_sort Sarfraz, Muhammad Hassan
collection PubMed
description The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of Trianthema portulacastrum. The synthesized nanoparticles were characterized using different techniques, such as the synthesis of the particles, which was confirmed by UV–visible spectrometry that showed absorbance at 300 nm, 255 nm, and 275 nm for the CH, CuO, and CH-CuO nanoparticles, respectively. The spherical morphology of the nanoparticles and the presence of active functional groups was validated by SEM, TEM, and FTIR analysis. The crystalline nature of the particles was verified by XRD spectrum, and the average crystallite sizes of 33.54 nm, 20.13 nm, and 24.14 nm were obtained, respectively. The characterized nanoparticles were evaluated for their in vitro antibacterial and antibiofilm potential against Acinetobacter baumannii isolates, where potent activities were exhibited by the nanoparticles. The bioassay for antioxidant activity also confirmed DPPH scavenging activity for all the nanoparticles. This study also evaluated anticancer activities of the CH, CuO, and CH-CuO nanoparticles against HepG2 cell lines, where maximum inhibitions of 54, 75, and 84% were recorded, respectively. The anticancer activity was also confirmed by phase contrast microscopy, where the treated cells exhibited deformed morphologies. This study demonstrates the potential of the CH-CuO nanoparticle as an effective antibacterial agent, having with its antibiofilm activity, and in cancer treatment.
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spelling pubmed-102645862023-06-15 Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines Sarfraz, Muhammad Hassan Zubair, Muhammad Aslam, Bilal Ashraf, Asma Siddique, Muhammad Hussnain Hayat, Sumreen Cruz, Jorrdy Neves Muzammil, Saima Khurshid, Mohsin Sarfraz, Muhammad Farrukh Hashem, Abeer Dawoud, Turki M. Avila-Quezada, Graciela Dolores Abd_Allah, Elsayed Fathi Front Microbiol Microbiology The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of Trianthema portulacastrum. The synthesized nanoparticles were characterized using different techniques, such as the synthesis of the particles, which was confirmed by UV–visible spectrometry that showed absorbance at 300 nm, 255 nm, and 275 nm for the CH, CuO, and CH-CuO nanoparticles, respectively. The spherical morphology of the nanoparticles and the presence of active functional groups was validated by SEM, TEM, and FTIR analysis. The crystalline nature of the particles was verified by XRD spectrum, and the average crystallite sizes of 33.54 nm, 20.13 nm, and 24.14 nm were obtained, respectively. The characterized nanoparticles were evaluated for their in vitro antibacterial and antibiofilm potential against Acinetobacter baumannii isolates, where potent activities were exhibited by the nanoparticles. The bioassay for antioxidant activity also confirmed DPPH scavenging activity for all the nanoparticles. This study also evaluated anticancer activities of the CH, CuO, and CH-CuO nanoparticles against HepG2 cell lines, where maximum inhibitions of 54, 75, and 84% were recorded, respectively. The anticancer activity was also confirmed by phase contrast microscopy, where the treated cells exhibited deformed morphologies. This study demonstrates the potential of the CH-CuO nanoparticle as an effective antibacterial agent, having with its antibiofilm activity, and in cancer treatment. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10264586/ /pubmed/37323910 http://dx.doi.org/10.3389/fmicb.2023.1188743 Text en Copyright © 2023 Sarfraz, Zubair, Aslam, Ashraf, Siddique, Hayat, Cruz, Muzammil, Khurshid, Sarfraz, Hashem, Dawoud, Avila-Quezada and Abd_Allah. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sarfraz, Muhammad Hassan
Zubair, Muhammad
Aslam, Bilal
Ashraf, Asma
Siddique, Muhammad Hussnain
Hayat, Sumreen
Cruz, Jorrdy Neves
Muzammil, Saima
Khurshid, Mohsin
Sarfraz, Muhammad Farrukh
Hashem, Abeer
Dawoud, Turki M.
Avila-Quezada, Graciela Dolores
Abd_Allah, Elsayed Fathi
Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title_full Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title_fullStr Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title_full_unstemmed Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title_short Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against Acinetobacter baumannii isolates and anticancer activity against HepG2 cell lines
title_sort comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based cuo nanoparticles: antibacterial potential against acinetobacter baumannii isolates and anticancer activity against hepg2 cell lines
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264586/
https://www.ncbi.nlm.nih.gov/pubmed/37323910
http://dx.doi.org/10.3389/fmicb.2023.1188743
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