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Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics
INTRODUCTION: Necrotizing enterocolitis (NEC) is a potentially fatal intestinal disease primarily affecting preterm infants. Early diagnosis of neonates with NEC is crucial to improving outcomes; however, traditional diagnostic tools remain inadequate. Biomarkers represent an opportunity to improve...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264655/ https://www.ncbi.nlm.nih.gov/pubmed/37325361 http://dx.doi.org/10.3389/fped.2023.1184940 |
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author | Mackay, Stephen Frazer, Lauren C. Bailey, Grace K. Miller, Claire M. Gong, Qingqing Dewitt, Olivia N. Singh, Dhirendra K. Good, Misty |
author_facet | Mackay, Stephen Frazer, Lauren C. Bailey, Grace K. Miller, Claire M. Gong, Qingqing Dewitt, Olivia N. Singh, Dhirendra K. Good, Misty |
author_sort | Mackay, Stephen |
collection | PubMed |
description | INTRODUCTION: Necrotizing enterocolitis (NEC) is a potentially fatal intestinal disease primarily affecting preterm infants. Early diagnosis of neonates with NEC is crucial to improving outcomes; however, traditional diagnostic tools remain inadequate. Biomarkers represent an opportunity to improve the speed and accuracy of diagnosis, but they are not routinely used in clinical practice. METHODS: In this study, we utilized an aptamer-based proteomic discovery assay to identify new serum biomarkers of NEC. We compared levels of serum proteins in neonates with and without NEC and identified ten differentially expressed serum proteins between these groups. RESULTS: We detected two proteins, C-C motif chemokine ligand 16 (CCL16) and immunoglobulin heavy constant alpha 1 and 2 heterodimer (IGHA1 IGHA2), that were significantly increased during NEC and eight that were significantly decreased. Generation of receiver operating characteristic (ROC) curves revealed that alpha-fetoprotein (AUC = 0.926), glucagon (AUC = 0.860), and IGHA1 IGHA2 (AUC = 0.826) were the proteins that best differentiated patients with and without NEC. DISCUSSION: These findings indicate that further investigation into these serum proteins as a biomarker for NEC is warranted. In the future, laboratory tests incorporating these differentially expressed proteins may improve the ability of clinicians to diagnose infants with NEC rapidly and accurately. |
format | Online Article Text |
id | pubmed-10264655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102646552023-06-15 Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics Mackay, Stephen Frazer, Lauren C. Bailey, Grace K. Miller, Claire M. Gong, Qingqing Dewitt, Olivia N. Singh, Dhirendra K. Good, Misty Front Pediatr Pediatrics INTRODUCTION: Necrotizing enterocolitis (NEC) is a potentially fatal intestinal disease primarily affecting preterm infants. Early diagnosis of neonates with NEC is crucial to improving outcomes; however, traditional diagnostic tools remain inadequate. Biomarkers represent an opportunity to improve the speed and accuracy of diagnosis, but they are not routinely used in clinical practice. METHODS: In this study, we utilized an aptamer-based proteomic discovery assay to identify new serum biomarkers of NEC. We compared levels of serum proteins in neonates with and without NEC and identified ten differentially expressed serum proteins between these groups. RESULTS: We detected two proteins, C-C motif chemokine ligand 16 (CCL16) and immunoglobulin heavy constant alpha 1 and 2 heterodimer (IGHA1 IGHA2), that were significantly increased during NEC and eight that were significantly decreased. Generation of receiver operating characteristic (ROC) curves revealed that alpha-fetoprotein (AUC = 0.926), glucagon (AUC = 0.860), and IGHA1 IGHA2 (AUC = 0.826) were the proteins that best differentiated patients with and without NEC. DISCUSSION: These findings indicate that further investigation into these serum proteins as a biomarker for NEC is warranted. In the future, laboratory tests incorporating these differentially expressed proteins may improve the ability of clinicians to diagnose infants with NEC rapidly and accurately. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10264655/ /pubmed/37325361 http://dx.doi.org/10.3389/fped.2023.1184940 Text en © 2023 Mackay, Frazer, Bailey, Miller, Gong, DeWitt, Singh and Good. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pediatrics Mackay, Stephen Frazer, Lauren C. Bailey, Grace K. Miller, Claire M. Gong, Qingqing Dewitt, Olivia N. Singh, Dhirendra K. Good, Misty Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title | Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title_full | Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title_fullStr | Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title_full_unstemmed | Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title_short | Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
title_sort | identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics |
topic | Pediatrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264655/ https://www.ncbi.nlm.nih.gov/pubmed/37325361 http://dx.doi.org/10.3389/fped.2023.1184940 |
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