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Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, th...

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Autores principales: Yang, Su, Chen, Jialuo, Ding, Yonghe, Huang, Qian, Chen, Guangna, Ulhassan, Zaid, Wei, Ji’an, Wang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264818/
https://www.ncbi.nlm.nih.gov/pubmed/37324688
http://dx.doi.org/10.3389/fpls.2023.1197781
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author Yang, Su
Chen, Jialuo
Ding, Yonghe
Huang, Qian
Chen, Guangna
Ulhassan, Zaid
Wei, Ji’an
Wang, Jian
author_facet Yang, Su
Chen, Jialuo
Ding, Yonghe
Huang, Qian
Chen, Guangna
Ulhassan, Zaid
Wei, Ji’an
Wang, Jian
author_sort Yang, Su
collection PubMed
description The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, there is a paucity of research investigating the role of the LOR gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 LOR genes in B. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 BnLORs could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 BnLOR members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that BnLORs involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of BnLOR family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate BnLOR gene expression under temperature, salinity and ABA stress, revealing that most BnLORs showed inducibility. This study enhanced our comprehension of the B. napus LOR gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.
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spelling pubmed-102648182023-06-15 Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress Yang, Su Chen, Jialuo Ding, Yonghe Huang, Qian Chen, Guangna Ulhassan, Zaid Wei, Ji’an Wang, Jian Front Plant Sci Plant Science The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, there is a paucity of research investigating the role of the LOR gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 LOR genes in B. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 BnLORs could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 BnLOR members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that BnLORs involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of BnLOR family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate BnLOR gene expression under temperature, salinity and ABA stress, revealing that most BnLORs showed inducibility. This study enhanced our comprehension of the B. napus LOR gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10264818/ /pubmed/37324688 http://dx.doi.org/10.3389/fpls.2023.1197781 Text en Copyright © 2023 Yang, Chen, Ding, Huang, Chen, Ulhassan, Wei and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Su
Chen, Jialuo
Ding, Yonghe
Huang, Qian
Chen, Guangna
Ulhassan, Zaid
Wei, Ji’an
Wang, Jian
Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title_full Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title_fullStr Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title_full_unstemmed Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title_short Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress
title_sort genome-wide investigation and expression profiling of lor gene family in rapeseed under salinity and aba stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264818/
https://www.ncbi.nlm.nih.gov/pubmed/37324688
http://dx.doi.org/10.3389/fpls.2023.1197781
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