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Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress

Citrus plants are sensitive to waterlogging, which can cause yield reduction. Their production heavily depends on the rootstock being used for grafting of scion cultivars, and the rootstock is the first organ to be affected by waterlogging stress. However, the underlying molecular mechanisms of wate...

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Autores principales: He, Wen, Luo, Liang, Xie, Rui, Chai, Jiufeng, Wang, Hao, Wang, Yan, Chen, Qing, Wu, Zhiwei, Yang, Shaofeng, Li, Mengyao, Lin, Yuanxiu, Zhang, Yunting, Luo, Ya, Zhang, Yong, Tang, Haoru, Wang, Xiaorong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264899/
https://www.ncbi.nlm.nih.gov/pubmed/37324702
http://dx.doi.org/10.3389/fpls.2023.1198930
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author He, Wen
Luo, Liang
Xie, Rui
Chai, Jiufeng
Wang, Hao
Wang, Yan
Chen, Qing
Wu, Zhiwei
Yang, Shaofeng
Li, Mengyao
Lin, Yuanxiu
Zhang, Yunting
Luo, Ya
Zhang, Yong
Tang, Haoru
Wang, Xiaorong
author_facet He, Wen
Luo, Liang
Xie, Rui
Chai, Jiufeng
Wang, Hao
Wang, Yan
Chen, Qing
Wu, Zhiwei
Yang, Shaofeng
Li, Mengyao
Lin, Yuanxiu
Zhang, Yunting
Luo, Ya
Zhang, Yong
Tang, Haoru
Wang, Xiaorong
author_sort He, Wen
collection PubMed
description Citrus plants are sensitive to waterlogging, which can cause yield reduction. Their production heavily depends on the rootstock being used for grafting of scion cultivars, and the rootstock is the first organ to be affected by waterlogging stress. However, the underlying molecular mechanisms of waterlogging stress tolerance remain elusive. In this study we investigated the stress response of two waterlogging-tolerant citrus varieties (Citrus junos Sieb ex Tanaka cv. Pujiang Xiangcheng and Ziyang Xiangcheng), and one waterlogging-sensitive variety (red tangerine) at the morphological, physiological, and genetic levels in leaf and root tissues of partially submerged plants. The results showed that waterlogging stress significantly decreased the SPAD value and root length but did not obviously affect the stem length and new root numbers. The malondialdehyde (MDA) content and the enzyme activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), and catalase (CAT) were enhanced in the roots. The RNA-seq analysis revealed that the differentially expressed genes (DEGs) were mainly linked to ‘cutin, suberine, and wax biosynthesis’, ‘diterpenoid biosynthesis’, and ‘glycerophospholipid metabolism’ in the leaves, whereas were linked to ‘flavonoid biosynthesis’, ‘biosynthesis of secondary metabolites and metabolic pathways’ in the roots. Finally, we developed a working model based on our results to elucidate the molecular basis of waterlogging-responsive in citrus. Therefore, our data obtained in this study provided valuable genetic resources that will facilitate the breeding of citrus varieties with improved waterlogging tolerance.
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spelling pubmed-102648992023-06-15 Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress He, Wen Luo, Liang Xie, Rui Chai, Jiufeng Wang, Hao Wang, Yan Chen, Qing Wu, Zhiwei Yang, Shaofeng Li, Mengyao Lin, Yuanxiu Zhang, Yunting Luo, Ya Zhang, Yong Tang, Haoru Wang, Xiaorong Front Plant Sci Plant Science Citrus plants are sensitive to waterlogging, which can cause yield reduction. Their production heavily depends on the rootstock being used for grafting of scion cultivars, and the rootstock is the first organ to be affected by waterlogging stress. However, the underlying molecular mechanisms of waterlogging stress tolerance remain elusive. In this study we investigated the stress response of two waterlogging-tolerant citrus varieties (Citrus junos Sieb ex Tanaka cv. Pujiang Xiangcheng and Ziyang Xiangcheng), and one waterlogging-sensitive variety (red tangerine) at the morphological, physiological, and genetic levels in leaf and root tissues of partially submerged plants. The results showed that waterlogging stress significantly decreased the SPAD value and root length but did not obviously affect the stem length and new root numbers. The malondialdehyde (MDA) content and the enzyme activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), and catalase (CAT) were enhanced in the roots. The RNA-seq analysis revealed that the differentially expressed genes (DEGs) were mainly linked to ‘cutin, suberine, and wax biosynthesis’, ‘diterpenoid biosynthesis’, and ‘glycerophospholipid metabolism’ in the leaves, whereas were linked to ‘flavonoid biosynthesis’, ‘biosynthesis of secondary metabolites and metabolic pathways’ in the roots. Finally, we developed a working model based on our results to elucidate the molecular basis of waterlogging-responsive in citrus. Therefore, our data obtained in this study provided valuable genetic resources that will facilitate the breeding of citrus varieties with improved waterlogging tolerance. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10264899/ /pubmed/37324702 http://dx.doi.org/10.3389/fpls.2023.1198930 Text en Copyright © 2023 He, Luo, Xie, Chai, Wang, Wang, Chen, Wu, Yang, Li, Lin, Zhang, Luo, Zhang, Tang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
He, Wen
Luo, Liang
Xie, Rui
Chai, Jiufeng
Wang, Hao
Wang, Yan
Chen, Qing
Wu, Zhiwei
Yang, Shaofeng
Li, Mengyao
Lin, Yuanxiu
Zhang, Yunting
Luo, Ya
Zhang, Yong
Tang, Haoru
Wang, Xiaorong
Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title_full Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title_fullStr Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title_full_unstemmed Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title_short Transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
title_sort transcriptome sequencing analyses uncover mechanisms of citrus rootstock seedlings under waterlogging stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264899/
https://www.ncbi.nlm.nih.gov/pubmed/37324702
http://dx.doi.org/10.3389/fpls.2023.1198930
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