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A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence

Millions of adenosine (A) to inosine (I) RNA editing events are reported and well‐studied in eukaryotes; however, many features and functions remain unclear in prokaryotes. By combining PacBio Sequel, Illumina whole‐genome sequencing, and RNA Sequencing data of two Klebsiella pneumoniae strains with...

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Autores principales: Yang, Xin‐Zhuang, Sun, Tian‐Shu, Jia, Pei‐Yao, Li, Sheng‐Jie, Li, Xiao‐Gang, Shi, Yanan, Li, Xue, Gao, Haotian, Yin, Huabing, Jia, Xin‐Miao, Yang, Qiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265045/
https://www.ncbi.nlm.nih.gov/pubmed/37083223
http://dx.doi.org/10.1002/advs.202206056
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author Yang, Xin‐Zhuang
Sun, Tian‐Shu
Jia, Pei‐Yao
Li, Sheng‐Jie
Li, Xiao‐Gang
Shi, Yanan
Li, Xue
Gao, Haotian
Yin, Huabing
Jia, Xin‐Miao
Yang, Qiwen
author_facet Yang, Xin‐Zhuang
Sun, Tian‐Shu
Jia, Pei‐Yao
Li, Sheng‐Jie
Li, Xiao‐Gang
Shi, Yanan
Li, Xue
Gao, Haotian
Yin, Huabing
Jia, Xin‐Miao
Yang, Qiwen
author_sort Yang, Xin‐Zhuang
collection PubMed
description Millions of adenosine (A) to inosine (I) RNA editing events are reported and well‐studied in eukaryotes; however, many features and functions remain unclear in prokaryotes. By combining PacBio Sequel, Illumina whole‐genome sequencing, and RNA Sequencing data of two Klebsiella pneumoniae strains with different virulence, a total of 13 RNA editing events are identified. The RNA editing event of badR is focused, which shows a significant difference in editing levels in the two K. pneumoniae strains and is predicted to be a transcription factor. A hard‐coded Cys is mutated on DNA to simulate the effect of complete editing of badR. Transcriptome analysis identifies the cellular quorum sensing (QS) pathway as the most dramatic change, demonstrating the dynamic regulation of RNA editing on badR related to coordinated collective behavior. Indeed, a significant difference in autoinducer 2 activity and cell growth is detected when the cells reach the stationary phase. Additionally, the mutant strain shows significantly lower virulence than the WT strain in the Galleria mellonella infection model. Furthermore, RNA editing regulation of badR is highly conserved across K. pneumoniae strains. Overall, this work provides new insights into posttranscriptional regulation in bacteria.
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spelling pubmed-102650452023-06-15 A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence Yang, Xin‐Zhuang Sun, Tian‐Shu Jia, Pei‐Yao Li, Sheng‐Jie Li, Xiao‐Gang Shi, Yanan Li, Xue Gao, Haotian Yin, Huabing Jia, Xin‐Miao Yang, Qiwen Adv Sci (Weinh) Research Articles Millions of adenosine (A) to inosine (I) RNA editing events are reported and well‐studied in eukaryotes; however, many features and functions remain unclear in prokaryotes. By combining PacBio Sequel, Illumina whole‐genome sequencing, and RNA Sequencing data of two Klebsiella pneumoniae strains with different virulence, a total of 13 RNA editing events are identified. The RNA editing event of badR is focused, which shows a significant difference in editing levels in the two K. pneumoniae strains and is predicted to be a transcription factor. A hard‐coded Cys is mutated on DNA to simulate the effect of complete editing of badR. Transcriptome analysis identifies the cellular quorum sensing (QS) pathway as the most dramatic change, demonstrating the dynamic regulation of RNA editing on badR related to coordinated collective behavior. Indeed, a significant difference in autoinducer 2 activity and cell growth is detected when the cells reach the stationary phase. Additionally, the mutant strain shows significantly lower virulence than the WT strain in the Galleria mellonella infection model. Furthermore, RNA editing regulation of badR is highly conserved across K. pneumoniae strains. Overall, this work provides new insights into posttranscriptional regulation in bacteria. John Wiley and Sons Inc. 2023-04-21 /pmc/articles/PMC10265045/ /pubmed/37083223 http://dx.doi.org/10.1002/advs.202206056 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yang, Xin‐Zhuang
Sun, Tian‐Shu
Jia, Pei‐Yao
Li, Sheng‐Jie
Li, Xiao‐Gang
Shi, Yanan
Li, Xue
Gao, Haotian
Yin, Huabing
Jia, Xin‐Miao
Yang, Qiwen
A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title_full A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title_fullStr A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title_full_unstemmed A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title_short A‐to‐I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence
title_sort a‐to‐i rna editing in klebsiella pneumoniae regulates quorum sensing and affects cell growth and virulence
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265045/
https://www.ncbi.nlm.nih.gov/pubmed/37083223
http://dx.doi.org/10.1002/advs.202206056
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