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Internal validation of an improved system for forensic application: a 41-plex Y-STR panel
: Y-chromosome short tandem repeats (Y-STRs) have a unique role in forensic investigation. However, low–medium mutating Y-STRs cannot meet the requirements for male lineage differentiation in inbred populations, whereas rapidly mutating (RM) high-resolution Y-STRs might cause unexpected exclusion o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265952/ https://www.ncbi.nlm.nih.gov/pubmed/37415794 http://dx.doi.org/10.1093/fsr/owad012 |
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author | Chai, Siyu Li, Min Tao, Ruiyang Xia, Ruocheng Kong, Qianqian Qu, Yiling Chen, Liqin Liu, Shiquan Li, Chengtao Chen, Pengyu Zhang, Suhua |
author_facet | Chai, Siyu Li, Min Tao, Ruiyang Xia, Ruocheng Kong, Qianqian Qu, Yiling Chen, Liqin Liu, Shiquan Li, Chengtao Chen, Pengyu Zhang, Suhua |
author_sort | Chai, Siyu |
collection | PubMed |
description | : Y-chromosome short tandem repeats (Y-STRs) have a unique role in forensic investigation. However, low–medium mutating Y-STRs cannot meet the requirements for male lineage differentiation in inbred populations, whereas rapidly mutating (RM) high-resolution Y-STRs might cause unexpected exclusion of paternal lineages. Thus, combining Y-STRs with low and high mutation rates helps to distinguish male individuals and lineages in family screening and analysis of genetic relationships. In this study, a novel 6-dye, 41-plex Y-STR panel was developed and validated, which included 17 loci from the Yfiler kit, nine RM Y-STR loci, 15 low–medium mutating Y-STR loci, and three Y-InDels. Developmental validation was performed for this panel, including size precision testing, stutter analysis, species specificity analysis, male specificity testing, sensitivity testing, concordance evaluation, polymerase chain reaction inhibitors analysis, and DNA mixture examination. The results demonstrated that the novel 41-plex Y-STR panel, developed in-house, was time efficient, accurate, and reliable. It showed good adaptability to directly amplify a variety of case-type samples. Furthermore, adding multiple Y-STR loci significantly improved the system’s ability to distinguish related males, making it highly informative for forensic applications. In addition, the data obtained were compatible with the widely used Y-STR kits, facilitating the search and construction of population databases. Moreover, the addition of Y-Indels with short amplicons improves the analyses of degraded samples. KEY POINTS: A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application. The multiplex included rapidly mutating Y-STRs and low–medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits. The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases. |
format | Online Article Text |
id | pubmed-10265952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102659522023-07-06 Internal validation of an improved system for forensic application: a 41-plex Y-STR panel Chai, Siyu Li, Min Tao, Ruiyang Xia, Ruocheng Kong, Qianqian Qu, Yiling Chen, Liqin Liu, Shiquan Li, Chengtao Chen, Pengyu Zhang, Suhua Forensic Sci Res Research Article : Y-chromosome short tandem repeats (Y-STRs) have a unique role in forensic investigation. However, low–medium mutating Y-STRs cannot meet the requirements for male lineage differentiation in inbred populations, whereas rapidly mutating (RM) high-resolution Y-STRs might cause unexpected exclusion of paternal lineages. Thus, combining Y-STRs with low and high mutation rates helps to distinguish male individuals and lineages in family screening and analysis of genetic relationships. In this study, a novel 6-dye, 41-plex Y-STR panel was developed and validated, which included 17 loci from the Yfiler kit, nine RM Y-STR loci, 15 low–medium mutating Y-STR loci, and three Y-InDels. Developmental validation was performed for this panel, including size precision testing, stutter analysis, species specificity analysis, male specificity testing, sensitivity testing, concordance evaluation, polymerase chain reaction inhibitors analysis, and DNA mixture examination. The results demonstrated that the novel 41-plex Y-STR panel, developed in-house, was time efficient, accurate, and reliable. It showed good adaptability to directly amplify a variety of case-type samples. Furthermore, adding multiple Y-STR loci significantly improved the system’s ability to distinguish related males, making it highly informative for forensic applications. In addition, the data obtained were compatible with the widely used Y-STR kits, facilitating the search and construction of population databases. Moreover, the addition of Y-Indels with short amplicons improves the analyses of degraded samples. KEY POINTS: A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application. The multiplex included rapidly mutating Y-STRs and low–medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits. The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases. Oxford University Press 2023-04-11 /pmc/articles/PMC10265952/ /pubmed/37415794 http://dx.doi.org/10.1093/fsr/owad012 Text en © The Author(s) 2023. Published by OUP on behalf of the Academy of Forensic Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chai, Siyu Li, Min Tao, Ruiyang Xia, Ruocheng Kong, Qianqian Qu, Yiling Chen, Liqin Liu, Shiquan Li, Chengtao Chen, Pengyu Zhang, Suhua Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title | Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title_full | Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title_fullStr | Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title_full_unstemmed | Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title_short | Internal validation of an improved system for forensic application: a 41-plex Y-STR panel |
title_sort | internal validation of an improved system for forensic application: a 41-plex y-str panel |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265952/ https://www.ncbi.nlm.nih.gov/pubmed/37415794 http://dx.doi.org/10.1093/fsr/owad012 |
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