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Quantification of gallium cryo-FIB milling damage in biological lamellae
Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-beam (F...
Autores principales: | Lucas, Bronwyn A., Grigorieff, Nikolaus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266028/ https://www.ncbi.nlm.nih.gov/pubmed/37216561 http://dx.doi.org/10.1073/pnas.2301852120 |
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