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Quantification of gallium cryo-FIB milling damage in biological lamellae

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-beam (F...

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Detalles Bibliográficos
Autores principales: Lucas, Bronwyn A., Grigorieff, Nikolaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266028/
https://www.ncbi.nlm.nih.gov/pubmed/37216561
http://dx.doi.org/10.1073/pnas.2301852120

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