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FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma
Objective: This study aimed to validate FANCI as a potential marker for both prognosis and therapy in liver hepatocellular carcinoma. Method: FANCI expression data were acquired from GEPIA, HPA, TCGA, and GEO databases. The impact of clinicopathological features was analyzed by UALCAN. The prognosis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266051/ https://www.ncbi.nlm.nih.gov/pubmed/37324186 http://dx.doi.org/10.7150/ijms.83760 |
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author | Hou, Yibo Li, Jianing Yu, Albert Deng, Kexin Chen, Jiawei Wang, Zixian Huang, Laiqiang Ma, Shaohua Dai, Xiaoyong |
author_facet | Hou, Yibo Li, Jianing Yu, Albert Deng, Kexin Chen, Jiawei Wang, Zixian Huang, Laiqiang Ma, Shaohua Dai, Xiaoyong |
author_sort | Hou, Yibo |
collection | PubMed |
description | Objective: This study aimed to validate FANCI as a potential marker for both prognosis and therapy in liver hepatocellular carcinoma. Method: FANCI expression data were acquired from GEPIA, HPA, TCGA, and GEO databases. The impact of clinicopathological features was analyzed by UALCAN. The prognosis of Liver Hepatocellular Carcinoma (LIHC) patients with highly expressed FANCI was constructed utilizing Kaplan-Meier Plotter. GEO2R was employed to identify differentially expressed genes (DEGs). Metascape was used to analyze functional pathways correlations. Protein-Protein interaction (PPI) networks were generated by Cytoscape. Furthermore, molecular complex detection (MCODE) was utilized to recognize Hub genes, which were selected to establish a prognostic model. Lastly, the relationship between FANCI and immune cell infiltration in LIHC was examined. Results: Compared to adjacent tissues, FANCI expression levels were significantly higher in LIHC tissues and were positively correlated to the cancer grade, stage, and prior hepatitis B virus (HBV) infection. High expression of FANCI was found to be associated with poor prognosis in LIHC (HR=1.89, p<0.001). DEGs that were positively correlated with FANCI were involved in various processes, including the cell cycle, VEGF pathway, immune system processes, and biogenesis of ribonucleoproteins. MCM10, TPX2, PRC1, and KIF11 were identified as key genes closely related to FANCI and poor prognosis. A reliable five-variable prognostic model was constructed with strong predictive capability. Lastly, a positive correlation was observed between FANCI expression and tumor-infiltration levels of CD8+ T cells, B cells, regulatory T (Tregs), CD4+ T helper 2 (Th2), and macrophage M2 cells. Conclusion: FANCI may hold promise as a potential biomarker for predicting prognostic outcomes, and a valuable therapeutic target for LIHC patients, with a focus on anti-proliferation, anti-chemoresistance, and combination with immunotherapy. |
format | Online Article Text |
id | pubmed-10266051 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-102660512023-06-15 FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma Hou, Yibo Li, Jianing Yu, Albert Deng, Kexin Chen, Jiawei Wang, Zixian Huang, Laiqiang Ma, Shaohua Dai, Xiaoyong Int J Med Sci Research Paper Objective: This study aimed to validate FANCI as a potential marker for both prognosis and therapy in liver hepatocellular carcinoma. Method: FANCI expression data were acquired from GEPIA, HPA, TCGA, and GEO databases. The impact of clinicopathological features was analyzed by UALCAN. The prognosis of Liver Hepatocellular Carcinoma (LIHC) patients with highly expressed FANCI was constructed utilizing Kaplan-Meier Plotter. GEO2R was employed to identify differentially expressed genes (DEGs). Metascape was used to analyze functional pathways correlations. Protein-Protein interaction (PPI) networks were generated by Cytoscape. Furthermore, molecular complex detection (MCODE) was utilized to recognize Hub genes, which were selected to establish a prognostic model. Lastly, the relationship between FANCI and immune cell infiltration in LIHC was examined. Results: Compared to adjacent tissues, FANCI expression levels were significantly higher in LIHC tissues and were positively correlated to the cancer grade, stage, and prior hepatitis B virus (HBV) infection. High expression of FANCI was found to be associated with poor prognosis in LIHC (HR=1.89, p<0.001). DEGs that were positively correlated with FANCI were involved in various processes, including the cell cycle, VEGF pathway, immune system processes, and biogenesis of ribonucleoproteins. MCM10, TPX2, PRC1, and KIF11 were identified as key genes closely related to FANCI and poor prognosis. A reliable five-variable prognostic model was constructed with strong predictive capability. Lastly, a positive correlation was observed between FANCI expression and tumor-infiltration levels of CD8+ T cells, B cells, regulatory T (Tregs), CD4+ T helper 2 (Th2), and macrophage M2 cells. Conclusion: FANCI may hold promise as a potential biomarker for predicting prognostic outcomes, and a valuable therapeutic target for LIHC patients, with a focus on anti-proliferation, anti-chemoresistance, and combination with immunotherapy. Ivyspring International Publisher 2023-05-12 /pmc/articles/PMC10266051/ /pubmed/37324186 http://dx.doi.org/10.7150/ijms.83760 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hou, Yibo Li, Jianing Yu, Albert Deng, Kexin Chen, Jiawei Wang, Zixian Huang, Laiqiang Ma, Shaohua Dai, Xiaoyong FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title | FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title_full | FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title_fullStr | FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title_full_unstemmed | FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title_short | FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma |
title_sort | fanci is associated with poor prognosis and immune infiltration in liver hepatocellular carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266051/ https://www.ncbi.nlm.nih.gov/pubmed/37324186 http://dx.doi.org/10.7150/ijms.83760 |
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