Cargando…

Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae

Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we e...

Descripción completa

Detalles Bibliográficos
Autores principales: Rocha, Natália Conceição, Lopes, Jessica Mariana, Russi, Karolayne Larissa, Palmeiro, Jussara Kasuko, Girardello, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266097/
https://www.ncbi.nlm.nih.gov/pubmed/37325517
http://dx.doi.org/10.3389/fcimb.2023.1139784
_version_ 1785058673727373312
author Rocha, Natália Conceição
Lopes, Jessica Mariana
Russi, Karolayne Larissa
Palmeiro, Jussara Kasuko
Girardello, Raquel
author_facet Rocha, Natália Conceição
Lopes, Jessica Mariana
Russi, Karolayne Larissa
Palmeiro, Jussara Kasuko
Girardello, Raquel
author_sort Rocha, Natália Conceição
collection PubMed
description Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we evaluated the accuracy of a commercial Policimbac® plate in determining the polymyxin B MIC for K. pneumoniae clinical isolates. The results were compared with those of the broth microdilution method according to ISO 16782. The Policimbac® plate had an excellent 98.04% categorical agreement, but unacceptable 31.37% essential agreement rates. Almost 2% of major errors as observed. Additionally, 52.94% of the strains overestimated the MIC at 1 µg/mL. Three isolates were excluded from the analysis due to the drying of the Policimbac® plate. To avoid dryness, we included wet gauze for the test, obtaining a 100% of categorical agreement rate; however, a low essential agreement was maintained (25.49%). In conclusion, the Policimbac® plate was unable to correctly determine the polymyxin B MIC for K. pneumoniae isolates. This low performance may interfere with the clinical use of the drug and, thus, with the result of the patient’s treatment.
format Online
Article
Text
id pubmed-10266097
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102660972023-06-15 Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae Rocha, Natália Conceição Lopes, Jessica Mariana Russi, Karolayne Larissa Palmeiro, Jussara Kasuko Girardello, Raquel Front Cell Infect Microbiol Cellular and Infection Microbiology Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we evaluated the accuracy of a commercial Policimbac® plate in determining the polymyxin B MIC for K. pneumoniae clinical isolates. The results were compared with those of the broth microdilution method according to ISO 16782. The Policimbac® plate had an excellent 98.04% categorical agreement, but unacceptable 31.37% essential agreement rates. Almost 2% of major errors as observed. Additionally, 52.94% of the strains overestimated the MIC at 1 µg/mL. Three isolates were excluded from the analysis due to the drying of the Policimbac® plate. To avoid dryness, we included wet gauze for the test, obtaining a 100% of categorical agreement rate; however, a low essential agreement was maintained (25.49%). In conclusion, the Policimbac® plate was unable to correctly determine the polymyxin B MIC for K. pneumoniae isolates. This low performance may interfere with the clinical use of the drug and, thus, with the result of the patient’s treatment. Frontiers Media S.A. 2023-05-30 /pmc/articles/PMC10266097/ /pubmed/37325517 http://dx.doi.org/10.3389/fcimb.2023.1139784 Text en Copyright © 2023 Rocha, Lopes, Russi, Palmeiro and Girardello https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rocha, Natália Conceição
Lopes, Jessica Mariana
Russi, Karolayne Larissa
Palmeiro, Jussara Kasuko
Girardello, Raquel
Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title_full Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title_fullStr Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title_full_unstemmed Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title_short Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
title_sort low performance of policimbac® broth microdilution in determining polymyxin b mic for klebsiella pneumoniae
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266097/
https://www.ncbi.nlm.nih.gov/pubmed/37325517
http://dx.doi.org/10.3389/fcimb.2023.1139784
work_keys_str_mv AT rochanataliaconceicao lowperformanceofpolicimbacbrothmicrodilutionindeterminingpolymyxinbmicforklebsiellapneumoniae
AT lopesjessicamariana lowperformanceofpolicimbacbrothmicrodilutionindeterminingpolymyxinbmicforklebsiellapneumoniae
AT russikarolaynelarissa lowperformanceofpolicimbacbrothmicrodilutionindeterminingpolymyxinbmicforklebsiellapneumoniae
AT palmeirojussarakasuko lowperformanceofpolicimbacbrothmicrodilutionindeterminingpolymyxinbmicforklebsiellapneumoniae
AT girardelloraquel lowperformanceofpolicimbacbrothmicrodilutionindeterminingpolymyxinbmicforklebsiellapneumoniae