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Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we e...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266097/ https://www.ncbi.nlm.nih.gov/pubmed/37325517 http://dx.doi.org/10.3389/fcimb.2023.1139784 |
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author | Rocha, Natália Conceição Lopes, Jessica Mariana Russi, Karolayne Larissa Palmeiro, Jussara Kasuko Girardello, Raquel |
author_facet | Rocha, Natália Conceição Lopes, Jessica Mariana Russi, Karolayne Larissa Palmeiro, Jussara Kasuko Girardello, Raquel |
author_sort | Rocha, Natália Conceição |
collection | PubMed |
description | Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we evaluated the accuracy of a commercial Policimbac® plate in determining the polymyxin B MIC for K. pneumoniae clinical isolates. The results were compared with those of the broth microdilution method according to ISO 16782. The Policimbac® plate had an excellent 98.04% categorical agreement, but unacceptable 31.37% essential agreement rates. Almost 2% of major errors as observed. Additionally, 52.94% of the strains overestimated the MIC at 1 µg/mL. Three isolates were excluded from the analysis due to the drying of the Policimbac® plate. To avoid dryness, we included wet gauze for the test, obtaining a 100% of categorical agreement rate; however, a low essential agreement was maintained (25.49%). In conclusion, the Policimbac® plate was unable to correctly determine the polymyxin B MIC for K. pneumoniae isolates. This low performance may interfere with the clinical use of the drug and, thus, with the result of the patient’s treatment. |
format | Online Article Text |
id | pubmed-10266097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102660972023-06-15 Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae Rocha, Natália Conceição Lopes, Jessica Mariana Russi, Karolayne Larissa Palmeiro, Jussara Kasuko Girardello, Raquel Front Cell Infect Microbiol Cellular and Infection Microbiology Klebsiella pneumoniae is a global threat to healthcare, and despite the availability of new drugs, polymyxins are still an important therapeutic option for this and other resistant gram-negative pathogens. Broth microdilution is the only method that is recommended for polymyxins. In this study, we evaluated the accuracy of a commercial Policimbac® plate in determining the polymyxin B MIC for K. pneumoniae clinical isolates. The results were compared with those of the broth microdilution method according to ISO 16782. The Policimbac® plate had an excellent 98.04% categorical agreement, but unacceptable 31.37% essential agreement rates. Almost 2% of major errors as observed. Additionally, 52.94% of the strains overestimated the MIC at 1 µg/mL. Three isolates were excluded from the analysis due to the drying of the Policimbac® plate. To avoid dryness, we included wet gauze for the test, obtaining a 100% of categorical agreement rate; however, a low essential agreement was maintained (25.49%). In conclusion, the Policimbac® plate was unable to correctly determine the polymyxin B MIC for K. pneumoniae isolates. This low performance may interfere with the clinical use of the drug and, thus, with the result of the patient’s treatment. Frontiers Media S.A. 2023-05-30 /pmc/articles/PMC10266097/ /pubmed/37325517 http://dx.doi.org/10.3389/fcimb.2023.1139784 Text en Copyright © 2023 Rocha, Lopes, Russi, Palmeiro and Girardello https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Rocha, Natália Conceição Lopes, Jessica Mariana Russi, Karolayne Larissa Palmeiro, Jussara Kasuko Girardello, Raquel Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae |
title | Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
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title_full | Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
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title_fullStr | Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
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title_full_unstemmed | Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
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title_short | Low performance of Policimbac® broth microdilution in determining polymyxin B MIC for Klebsiella pneumoniae
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title_sort | low performance of policimbac® broth microdilution in determining polymyxin b mic for klebsiella pneumoniae |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266097/ https://www.ncbi.nlm.nih.gov/pubmed/37325517 http://dx.doi.org/10.3389/fcimb.2023.1139784 |
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