Cargando…

A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage

Objective: Developing a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA), which does not require any complicated instruments and can be visually co...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xinyang, Li, Miyang, Duan, Shengbao, Wang, Hongmei, Li, Yong, Liu, Tiemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266101/
https://www.ncbi.nlm.nih.gov/pubmed/37324444
http://dx.doi.org/10.3389/fbioe.2023.1175345
_version_ 1785058674728763392
author Li, Xinyang
Li, Miyang
Duan, Shengbao
Wang, Hongmei
Li, Yong
Liu, Tiemei
author_facet Li, Xinyang
Li, Miyang
Duan, Shengbao
Wang, Hongmei
Li, Yong
Liu, Tiemei
author_sort Li, Xinyang
collection PubMed
description Objective: Developing a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA), which does not require any complicated instruments and can be visually colored, so as to provide a new method for clinical detection of fetomaternal haemorrhage. Methods: As a carrier, a chemically treated silk membrane was used to immobilize anti-A/anti-B antibody reagent. PBS washed slowly after vertically dropping red blood cells. After adding biotin-labeled anti-A/anti-B antibody reagent, PBS is slowly washed, enzyme-labeled avidin is added, and TMB is used for color development after washing. Results: When there were both anti-A and anti-B fetal erythrocytes in pregnant women’s peripheral blood, the final color was dark brown. When there are no anti-A and anti-B fetal red blood cells in pregnant women’s peripheral blood, the final color development results do not change, which corresponds to the color of chemically treated silk membrane. Conclusion: The new enzyme-linked immunosorbent assay (ELISA) based on a silk membrane can distinguish fetal red blood cells from maternal red blood cells prenatally and can be used for prenatal detection of fetomaternal haemorrhage.
format Online
Article
Text
id pubmed-10266101
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102661012023-06-15 A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage Li, Xinyang Li, Miyang Duan, Shengbao Wang, Hongmei Li, Yong Liu, Tiemei Front Bioeng Biotechnol Bioengineering and Biotechnology Objective: Developing a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA), which does not require any complicated instruments and can be visually colored, so as to provide a new method for clinical detection of fetomaternal haemorrhage. Methods: As a carrier, a chemically treated silk membrane was used to immobilize anti-A/anti-B antibody reagent. PBS washed slowly after vertically dropping red blood cells. After adding biotin-labeled anti-A/anti-B antibody reagent, PBS is slowly washed, enzyme-labeled avidin is added, and TMB is used for color development after washing. Results: When there were both anti-A and anti-B fetal erythrocytes in pregnant women’s peripheral blood, the final color was dark brown. When there are no anti-A and anti-B fetal red blood cells in pregnant women’s peripheral blood, the final color development results do not change, which corresponds to the color of chemically treated silk membrane. Conclusion: The new enzyme-linked immunosorbent assay (ELISA) based on a silk membrane can distinguish fetal red blood cells from maternal red blood cells prenatally and can be used for prenatal detection of fetomaternal haemorrhage. Frontiers Media S.A. 2023-05-26 /pmc/articles/PMC10266101/ /pubmed/37324444 http://dx.doi.org/10.3389/fbioe.2023.1175345 Text en Copyright © 2023 Li, Li, Duan, Wang, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Xinyang
Li, Miyang
Duan, Shengbao
Wang, Hongmei
Li, Yong
Liu, Tiemei
A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title_full A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title_fullStr A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title_full_unstemmed A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title_short A novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
title_sort novel enzyme-linked immunostaining technique based on silk membrane for the prenatal detection of fetomaternal haemorrhage
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266101/
https://www.ncbi.nlm.nih.gov/pubmed/37324444
http://dx.doi.org/10.3389/fbioe.2023.1175345
work_keys_str_mv AT lixinyang anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT limiyang anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT duanshengbao anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT wanghongmei anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT liyong anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT liutiemei anovelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT lixinyang novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT limiyang novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT duanshengbao novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT wanghongmei novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT liyong novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage
AT liutiemei novelenzymelinkedimmunostainingtechniquebasedonsilkmembranefortheprenataldetectionoffetomaternalhaemorrhage