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Transcriptome profiling of the initial segment and proximal caput of mouse epididymis
BACKGROUND: The proximal region of the mouse epididymis plays a pivotal role in sperm transport, sperm maturation, and male fertility. Several studies have focused on segment-dependent gene expression of the mouse epididymis through high-throughput sequencing without the precision of the microdissec...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266198/ https://www.ncbi.nlm.nih.gov/pubmed/37324270 http://dx.doi.org/10.3389/fendo.2023.1190890 |
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author | Wang, Xiao Qiu, Fanyi Yu, Junjie Zhou, Meiyang Zuo, Anjian Xu, Xiaojiang Sun, Xiao-Yang Wang, Zhengpin |
author_facet | Wang, Xiao Qiu, Fanyi Yu, Junjie Zhou, Meiyang Zuo, Anjian Xu, Xiaojiang Sun, Xiao-Yang Wang, Zhengpin |
author_sort | Wang, Xiao |
collection | PubMed |
description | BACKGROUND: The proximal region of the mouse epididymis plays a pivotal role in sperm transport, sperm maturation, and male fertility. Several studies have focused on segment-dependent gene expression of the mouse epididymis through high-throughput sequencing without the precision of the microdissection. METHODS AND RESULTS: Herein, we isolated the initial segment (IS) and proximal caput (P-caput) by physical microdissection using an Lcn9-cre; Rosa26(tdTomato) mouse model. We defined the transcriptome changes of caput epididymis by RNA sequencing (RNA-seq), which identified 1,961 genes that were abundantly expressed in the IS and 1,739 genes that were prominently expressed in the P-caput. In addition, we found that many differentially expressed genes (DEGs) were predominantly or uniquely expressed in the epididymis and region-specific genes were highly associated with transport, secretion, sperm motility, fertilization, and male fertility. CONCLUSION: Thus, this study provides an RNA-seq resource to identify region-specific genes in the caput epididymis. The epididymal-selective/specific genes are potential targets for male contraception and may provide new insights into understanding segment-specific epididymal microenvironment-mediated sperm transport, maturation, and male fertility. |
format | Online Article Text |
id | pubmed-10266198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102661982023-06-15 Transcriptome profiling of the initial segment and proximal caput of mouse epididymis Wang, Xiao Qiu, Fanyi Yu, Junjie Zhou, Meiyang Zuo, Anjian Xu, Xiaojiang Sun, Xiao-Yang Wang, Zhengpin Front Endocrinol (Lausanne) Endocrinology BACKGROUND: The proximal region of the mouse epididymis plays a pivotal role in sperm transport, sperm maturation, and male fertility. Several studies have focused on segment-dependent gene expression of the mouse epididymis through high-throughput sequencing without the precision of the microdissection. METHODS AND RESULTS: Herein, we isolated the initial segment (IS) and proximal caput (P-caput) by physical microdissection using an Lcn9-cre; Rosa26(tdTomato) mouse model. We defined the transcriptome changes of caput epididymis by RNA sequencing (RNA-seq), which identified 1,961 genes that were abundantly expressed in the IS and 1,739 genes that were prominently expressed in the P-caput. In addition, we found that many differentially expressed genes (DEGs) were predominantly or uniquely expressed in the epididymis and region-specific genes were highly associated with transport, secretion, sperm motility, fertilization, and male fertility. CONCLUSION: Thus, this study provides an RNA-seq resource to identify region-specific genes in the caput epididymis. The epididymal-selective/specific genes are potential targets for male contraception and may provide new insights into understanding segment-specific epididymal microenvironment-mediated sperm transport, maturation, and male fertility. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10266198/ /pubmed/37324270 http://dx.doi.org/10.3389/fendo.2023.1190890 Text en Copyright © 2023 Wang, Qiu, Yu, Zhou, Zuo, Xu, Sun and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Wang, Xiao Qiu, Fanyi Yu, Junjie Zhou, Meiyang Zuo, Anjian Xu, Xiaojiang Sun, Xiao-Yang Wang, Zhengpin Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title | Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title_full | Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title_fullStr | Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title_full_unstemmed | Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title_short | Transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
title_sort | transcriptome profiling of the initial segment and proximal caput of mouse epididymis |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266198/ https://www.ncbi.nlm.nih.gov/pubmed/37324270 http://dx.doi.org/10.3389/fendo.2023.1190890 |
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