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In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds

Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hy...

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Autores principales: Li, Sipan, Wang, Yongan, Wang, Siting, Xie, Jianjun, Fu, Tingming, Li, Shaoguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266532/
https://www.ncbi.nlm.nih.gov/pubmed/37324421
http://dx.doi.org/10.3389/fbioe.2023.1189010
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author Li, Sipan
Wang, Yongan
Wang, Siting
Xie, Jianjun
Fu, Tingming
Li, Shaoguang
author_facet Li, Sipan
Wang, Yongan
Wang, Siting
Xie, Jianjun
Fu, Tingming
Li, Shaoguang
author_sort Li, Sipan
collection PubMed
description Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice. Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days. Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group. Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections.
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spelling pubmed-102665322023-06-15 In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds Li, Sipan Wang, Yongan Wang, Siting Xie, Jianjun Fu, Tingming Li, Shaoguang Front Bioeng Biotechnol Bioengineering and Biotechnology Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice. Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days. Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group. Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections. Frontiers Media S.A. 2023-05-31 /pmc/articles/PMC10266532/ /pubmed/37324421 http://dx.doi.org/10.3389/fbioe.2023.1189010 Text en Copyright © 2023 Li, Wang, Wang, Xie, Fu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Sipan
Wang, Yongan
Wang, Siting
Xie, Jianjun
Fu, Tingming
Li, Shaoguang
In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title_full In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title_fullStr In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title_full_unstemmed In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title_short In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
title_sort in situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266532/
https://www.ncbi.nlm.nih.gov/pubmed/37324421
http://dx.doi.org/10.3389/fbioe.2023.1189010
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