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Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon
Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), an exogenous cytokinin (CK) growth regulator, is the conventional method for inducing fruit set during melon (Cucumis melo L.) production; however, the mechanism by which CPPU induces fruit set is unclear. Through histological and morphological o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266944/ https://www.ncbi.nlm.nih.gov/pubmed/37323228 http://dx.doi.org/10.1093/hr/uhad084 |
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author | Liu, Yue Li, Yang Guo, Huixin Lv, Bingsheng Feng, Jing Wang, Huihui Zhang, Zhonghua Chai, Sen |
author_facet | Liu, Yue Li, Yang Guo, Huixin Lv, Bingsheng Feng, Jing Wang, Huihui Zhang, Zhonghua Chai, Sen |
author_sort | Liu, Yue |
collection | PubMed |
description | Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), an exogenous cytokinin (CK) growth regulator, is the conventional method for inducing fruit set during melon (Cucumis melo L.) production; however, the mechanism by which CPPU induces fruit set is unclear. Through histological and morphological observations, fruit size was comparable between CPPU-induced fruits and normal pollinated fruits because CPPU-induced fruits had higher cell density but smaller cell size compared with normal pollinated fruits. CPPU promotes the accumulation of gibberellin (GA) and auxin and decreases the level of abscisic acid (ABA) during fruit set. Moreover, application of the GA inhibitor paclobutrazol (PAC) partially inhibits CPPU-induced fruit set. Transcriptome analysis revealed that CPPU-induced fruit set specifically induced the GA-related pathway, in which the key synthase encoding gibberellin 20-oxidase 1 (CmGA20ox1) was specifically upregulated. Further study indicated that the two-component response regulator 2 (CmRR2) of the cytokinin signaling pathway, which is highly expressed at fruit setting, positively regulates the expression of CmGA20ox1. Collectively, our study determined that CPPU-induced melon fruit set is dependent on GA biosynthesis, providing a theoretical basis for the creation of parthenocarpic melon germplasm. |
format | Online Article Text |
id | pubmed-10266944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102669442023-06-15 Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon Liu, Yue Li, Yang Guo, Huixin Lv, Bingsheng Feng, Jing Wang, Huihui Zhang, Zhonghua Chai, Sen Hortic Res Article Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), an exogenous cytokinin (CK) growth regulator, is the conventional method for inducing fruit set during melon (Cucumis melo L.) production; however, the mechanism by which CPPU induces fruit set is unclear. Through histological and morphological observations, fruit size was comparable between CPPU-induced fruits and normal pollinated fruits because CPPU-induced fruits had higher cell density but smaller cell size compared with normal pollinated fruits. CPPU promotes the accumulation of gibberellin (GA) and auxin and decreases the level of abscisic acid (ABA) during fruit set. Moreover, application of the GA inhibitor paclobutrazol (PAC) partially inhibits CPPU-induced fruit set. Transcriptome analysis revealed that CPPU-induced fruit set specifically induced the GA-related pathway, in which the key synthase encoding gibberellin 20-oxidase 1 (CmGA20ox1) was specifically upregulated. Further study indicated that the two-component response regulator 2 (CmRR2) of the cytokinin signaling pathway, which is highly expressed at fruit setting, positively regulates the expression of CmGA20ox1. Collectively, our study determined that CPPU-induced melon fruit set is dependent on GA biosynthesis, providing a theoretical basis for the creation of parthenocarpic melon germplasm. Oxford University Press 2023-05-03 /pmc/articles/PMC10266944/ /pubmed/37323228 http://dx.doi.org/10.1093/hr/uhad084 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Liu, Yue Li, Yang Guo, Huixin Lv, Bingsheng Feng, Jing Wang, Huihui Zhang, Zhonghua Chai, Sen Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title_full | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title_fullStr | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title_full_unstemmed | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title_short | Gibberellin biosynthesis is required for CPPU-induced parthenocarpy in melon |
title_sort | gibberellin biosynthesis is required for cppu-induced parthenocarpy in melon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266944/ https://www.ncbi.nlm.nih.gov/pubmed/37323228 http://dx.doi.org/10.1093/hr/uhad084 |
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