Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on the transport of a bifunctional substrate comprised of an antigen fused to chlora...

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Detalles Bibliográficos
Autores principales: Robinson, Michael-Paul, Jung, Jinjoo, Lopez-Barbosa, Natalia, Chang, Matthew, Li, Mingji, Jaroentomeechai, Thapakorn, Cox, Emily C., Zheng, Xiaolu, Berkmen, Mehmet, DeLisa, Matthew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267130/
https://www.ncbi.nlm.nih.gov/pubmed/37316535
http://dx.doi.org/10.1038/s41467-023-39178-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267130/
https://www.ncbi.nlm.nih.gov/pubmed/37316535
http://dx.doi.org/10.1038/s41467-023-39178-x

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