Cargando…

Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on the transport of a bifunctional substrate comprised of an antigen fused to chlora...

Descripción completa

Detalles Bibliográficos
Autores principales:	Robinson, Michael-Paul, Jung, Jinjoo, Lopez-Barbosa, Natalia, Chang, Matthew, Li, Mingji, Jaroentomeechai, Thapakorn, Cox, Emily C., Zheng, Xiaolu, Berkmen, Mehmet, DeLisa, Matthew P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267130/ https://www.ncbi.nlm.nih.gov/pubmed/37316535 http://dx.doi.org/10.1038/s41467-023-39178-x

Ejemplares similares

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria
por: Robinson, Michael-Paul, et al.
Publicado: (2015)

Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells
por: Jaroentomeechai, Thapakorn, et al.
Publicado: (2020)

Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteins
por: Li, Mingji, et al.
Publicado: (2021)

A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
por: Jaroentomeechai, Thapakorn, et al.
Publicado: (2022)

Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences
por: Ollis, Anne A., et al.
Publicado: (2015)

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
por: Jaroentomeechai, Thapakorn, et al.
Publicado: (2018)

Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
por: Jaroentomeechai, Thapakorn, et al.
Publicado: (2018)

Hinge length contributes to the phagocytic activity of HIV-specific IgG1 and IgG3 antibodies
por: Chu, Thach H., et al.
Publicado: (2020)

Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
por: Chan, Conrad En Zuo, et al.
Publicado: (2010)

Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag
por: Ke, Na, et al.
Publicado: (2016)

Multifunctional IgG/IgM antibodies and cellular cytotoxicity are elicited by the full-length MSP1 SumayaVac-1 malaria vaccine
por: Rosenkranz, Micha, et al.
Publicado: (2023)

A Novel Full-length IgG Recombinant Antibody Highly Specific to Clothianidin and Its Application in Immunochromatographic Assay
por: Chang, Yunyun, et al.
Publicado: (2022)

Investigation of interactions between binding residues and solubility of grafted humanized anti-VEGF IgG antibodies expressed as full-length format in the cytoplasm of a novel engineered E. coli SHuffle strain
por: Sangpheak, Kanyani, et al.
Publicado: (2021)

IgG4-related hypertrophic pachymeningitis coexpressing antineutrophil cytoplasmic antibodies
por: Massey, Jennifer
Publicado: (2017)

A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo
por: Mizrachi, Dario, et al.
Publicado: (2017)

Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins
por: Fang, Yongliang, et al.
Publicado: (2017)

Hypertrophic pachymeningitis IgG4-related with antineutrophil cytoplasmic antibody positivity and aortitis
por: Musto, Alessia, et al.
Publicado: (2019)

Development and Optimization of In-house ELISA for Detection of Human IgG Antibody to SARS-CoV-2 Full Length Spike Protein
por: Alandijany, Thamir A., et al.
Publicado: (2020)

Subclasses of allergen-specific IgG: Serum IgG2 and IgG3 levels are not predicted by IgG1/IgG4 levels
por: MacGlashan, Donald, et al.
Publicado: (2021)

Pacific Biosciences Sequencing and IMGT/HighV-QUEST Analysis of Full-Length Single Chain Fragment Variable from an In Vivo Selected Phage-Display Combinatorial Library
por: Hemadou, Audrey, et al.
Publicado: (2017)

First steps in the standardization of immunoglobulin IgG myeloperoxidase‐anti‐neutrophil cytoplasmic antibody measurements
por: Hutu, D. P., et al.
Publicado: (2015)

YEAST SURFACE DISPLAY OF FULL-LENGTH IGG FOR ENGINEERING MONOCLONAL AND BISPECIFIC ANTIBODIES
por: Jiang, Youwei
Publicado: (2023)

Concurrent IgG4-related tubulointerstitial nephritis and IgG4 myeloperoxidase-anti-neutrophil cytoplasmic antibody positive crescentic glomerulonephritis: A case report
por: Su, Tao, et al.
Publicado: (2017)

The Cytoplasmic Tail of FPC Antagonizes the Full-Length Protein in the Regulation of mTOR Pathway
por: Wang, Shixuan, et al.
Publicado: (2014)

On-demand biomanufacturing of protective conjugate vaccines
por: Stark, Jessica C., et al.
Publicado: (2021)

Mast Cells Exhibiting Strong Cytoplasmic Staining for IgE and High Affinity IgE Receptor are Increased in IgG4-Related Disease
por: Nishida, Kenji, et al.
Publicado: (2018)

Toward in Silico Modeling of Dynamic Combinatorial Libraries
por: Casciuc, Iuri, et al.
Publicado: (2022)

The antiinflammatory activity of IgG: the intravenous IgG paradox
por: Nimmerjahn, Falk, et al.
Publicado: (2007)

Serum IgG4 levels outperform IgG4/IgG RNA ratio in differential diagnosis of IgG4-related disease
por: Schulte, Lucas A., et al.
Publicado: (2020)

Significance of Urinary Full-Length Megalin in Patients with IgA Nephropathy
por: Seki, Takuto, et al.
Publicado: (2014)

Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)
por: Blair, Matthew W, et al.
Publicado: (2011)

IgGs drive COVID-19 myeloid hyperinflammation
por: Park, Matthew D.
Publicado: (2020)

Enriched blood IgG sialylation attenuates IgG-mediated and IgG-controlled-IgE-mediated allergic reactions
por: Petry, Janina, et al.
Publicado: (2020)

Association of dengue infection with anti-alpha-gal antibodies, IgM, IgG, IgG1, and IgG2
por: Olajiga, Olayinka M., et al.
Publicado: (2022)

Penalized likelihood for sparse contingency tables with an application to full-length cDNA libraries
por: Dahinden, Corinne, et al.
Publicado: (2007)

Full-Length Enrich c-DNA Libraries-Clear Cell-Renal Cell Carcinoma
por: Tang, Sai-Wen, et al.
Publicado: (2012)

cDNA Library Enrichment of Full Length Transcripts for SMRT Long Read Sequencing
por: Cartolano, Maria, et al.
Publicado: (2016)

Generation of full-length circular RNA libraries for Oxford Nanopore long-read sequencing
por: Fuchs, Steffen, et al.
Publicado: (2022)

Nonameric structures of the cytoplasmic domain of FlhA and SctV in the context of the full-length protein
por: Kuhlen, Lucas, et al.
Publicado: (2021)

Specificity of fc receptors for IgG2a, IgG1/IgG2b, and IgE on rat macrophages
Publicado: (1981)

Cannot write session to /tmp/vufind_sessions/sess_fb4nib6eq1u7eeuuqd5jo5ls47