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Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Leaf color mutants are important materials for studying chloroplast and photomorphogenesis, and can function as basic germplasms for genetic breeding. In an ethylmethanesulfonate mutagenesis population of watermelon cultivar “703”, a chlorophyll-deficient mutant with yellow leaf (Yl2) color was iden...

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Autores principales: Xu, Binghua, Zhang, Chaoyang, Gu, Yan, Cheng, Rui, Huang, Dayue, Liu, Xin, Sun, Yudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267204/
https://www.ncbi.nlm.nih.gov/pubmed/37316569
http://dx.doi.org/10.1038/s41598-023-36656-6
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author Xu, Binghua
Zhang, Chaoyang
Gu, Yan
Cheng, Rui
Huang, Dayue
Liu, Xin
Sun, Yudong
author_facet Xu, Binghua
Zhang, Chaoyang
Gu, Yan
Cheng, Rui
Huang, Dayue
Liu, Xin
Sun, Yudong
author_sort Xu, Binghua
collection PubMed
description Leaf color mutants are important materials for studying chloroplast and photomorphogenesis, and can function as basic germplasms for genetic breeding. In an ethylmethanesulfonate mutagenesis population of watermelon cultivar “703”, a chlorophyll-deficient mutant with yellow leaf (Yl2) color was identified. The contents of chlorophyll a, chlorophyll b, and carotenoids in Yl2 leaves were lower than those in wild-type (WT) leaves. The chloroplast ultrastructure in the leaves revealed that the chloroplasts in Yl2 were degraded. The numbers of chloroplasts and thylakoids in the Yl2 mutant were lower, resulting in lower photosynthetic parameters. Transcriptomic analysis identified 1292 differentially expressed genes, including1002 upregulated and 290 downregulated genes. The genes involved in chlorophyll biosynthesis (HEMA, HEMD, CHL1, CHLM, and CAO) were significantly downregulated in the Yl2 mutant, which may explain why chlorophyll pigment content was lower than that in the WT. Chlorophyll metabolism genes such as PDS, ZDS and VDE, were upregulated, which form the xanthophyll cycle and may protect the yellow‒leaves plants from photodamage. Taken together, our findings provide insight into the molecular mechanisms of leading to leaf color formation and chloroplast development in watermelon.
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spelling pubmed-102672042023-06-15 Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon Xu, Binghua Zhang, Chaoyang Gu, Yan Cheng, Rui Huang, Dayue Liu, Xin Sun, Yudong Sci Rep Article Leaf color mutants are important materials for studying chloroplast and photomorphogenesis, and can function as basic germplasms for genetic breeding. In an ethylmethanesulfonate mutagenesis population of watermelon cultivar “703”, a chlorophyll-deficient mutant with yellow leaf (Yl2) color was identified. The contents of chlorophyll a, chlorophyll b, and carotenoids in Yl2 leaves were lower than those in wild-type (WT) leaves. The chloroplast ultrastructure in the leaves revealed that the chloroplasts in Yl2 were degraded. The numbers of chloroplasts and thylakoids in the Yl2 mutant were lower, resulting in lower photosynthetic parameters. Transcriptomic analysis identified 1292 differentially expressed genes, including1002 upregulated and 290 downregulated genes. The genes involved in chlorophyll biosynthesis (HEMA, HEMD, CHL1, CHLM, and CAO) were significantly downregulated in the Yl2 mutant, which may explain why chlorophyll pigment content was lower than that in the WT. Chlorophyll metabolism genes such as PDS, ZDS and VDE, were upregulated, which form the xanthophyll cycle and may protect the yellow‒leaves plants from photodamage. Taken together, our findings provide insight into the molecular mechanisms of leading to leaf color formation and chloroplast development in watermelon. Nature Publishing Group UK 2023-06-14 /pmc/articles/PMC10267204/ /pubmed/37316569 http://dx.doi.org/10.1038/s41598-023-36656-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Xu, Binghua
Zhang, Chaoyang
Gu, Yan
Cheng, Rui
Huang, Dayue
Liu, Xin
Sun, Yudong
Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title_full Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title_fullStr Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title_full_unstemmed Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title_short Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
title_sort physiological and transcriptomic analysis of a yellow leaf mutant in watermelon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267204/
https://www.ncbi.nlm.nih.gov/pubmed/37316569
http://dx.doi.org/10.1038/s41598-023-36656-6
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