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CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment

Immune response is an important factor in determining the fate of bone replacement materials, in which macrophages play an important role. It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization...

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Autores principales: Qiang, Huifen, Hou, Caiyao, Zhang, Yujue, Luo, Xin, Li, Jun, Meng, Chunxiu, Liu, Kun, Lv, Zhaoyong, Chen, Ximeng, Liu, Fengzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267298/
https://www.ncbi.nlm.nih.gov/pubmed/37324238
http://dx.doi.org/10.1093/rb/rbad051
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author Qiang, Huifen
Hou, Caiyao
Zhang, Yujue
Luo, Xin
Li, Jun
Meng, Chunxiu
Liu, Kun
Lv, Zhaoyong
Chen, Ximeng
Liu, Fengzhen
author_facet Qiang, Huifen
Hou, Caiyao
Zhang, Yujue
Luo, Xin
Li, Jun
Meng, Chunxiu
Liu, Kun
Lv, Zhaoyong
Chen, Ximeng
Liu, Fengzhen
author_sort Qiang, Huifen
collection PubMed
description Immune response is an important factor in determining the fate of bone replacement materials, in which macrophages play an important role. It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization. In this work, the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated. We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation, which could effectively upregulate osteogenesis-related factors and promote new bone formation, indicating the important role of macrophages polarization in biomaterial induction of osteogenesis. In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation. In addition, transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages, activating Toll-like receptor signaling pathway, which participated in the activation and attenuation of inflammation, and accelerated bone integration. Thus, by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release, the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration.
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spelling pubmed-102672982023-06-15 CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment Qiang, Huifen Hou, Caiyao Zhang, Yujue Luo, Xin Li, Jun Meng, Chunxiu Liu, Kun Lv, Zhaoyong Chen, Ximeng Liu, Fengzhen Regen Biomater Research Article Immune response is an important factor in determining the fate of bone replacement materials, in which macrophages play an important role. It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization. In this work, the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated. We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation, which could effectively upregulate osteogenesis-related factors and promote new bone formation, indicating the important role of macrophages polarization in biomaterial induction of osteogenesis. In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation. In addition, transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages, activating Toll-like receptor signaling pathway, which participated in the activation and attenuation of inflammation, and accelerated bone integration. Thus, by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release, the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration. Oxford University Press 2023-05-09 /pmc/articles/PMC10267298/ /pubmed/37324238 http://dx.doi.org/10.1093/rb/rbad051 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qiang, Huifen
Hou, Caiyao
Zhang, Yujue
Luo, Xin
Li, Jun
Meng, Chunxiu
Liu, Kun
Lv, Zhaoyong
Chen, Ximeng
Liu, Fengzhen
CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title_full CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title_fullStr CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title_full_unstemmed CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title_short CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
title_sort cap-coated zn-mn-li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267298/
https://www.ncbi.nlm.nih.gov/pubmed/37324238
http://dx.doi.org/10.1093/rb/rbad051
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