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Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering

Mimicking bone extracellular matrix (ECM) is paramount to develop novel biomaterials for bone tissue engineering. In this regard, the combination of integrin-binding ligands together with osteogenic peptides represents a powerful approach to recapitulate the healing microenvironment of bone. In the...

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Autores principales: Oliver-Cervelló, Lluís, Martin-Gómez, Helena, Gonzalez-Garcia, Cristina, Salmeron-Sanchez, Manuel, Ginebra, Maria-Pau, Mas-Moruno, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267393/
https://www.ncbi.nlm.nih.gov/pubmed/37324414
http://dx.doi.org/10.3389/fbioe.2023.1192436
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author Oliver-Cervelló, Lluís
Martin-Gómez, Helena
Gonzalez-Garcia, Cristina
Salmeron-Sanchez, Manuel
Ginebra, Maria-Pau
Mas-Moruno, Carlos
author_facet Oliver-Cervelló, Lluís
Martin-Gómez, Helena
Gonzalez-Garcia, Cristina
Salmeron-Sanchez, Manuel
Ginebra, Maria-Pau
Mas-Moruno, Carlos
author_sort Oliver-Cervelló, Lluís
collection PubMed
description Mimicking bone extracellular matrix (ECM) is paramount to develop novel biomaterials for bone tissue engineering. In this regard, the combination of integrin-binding ligands together with osteogenic peptides represents a powerful approach to recapitulate the healing microenvironment of bone. In the present work, we designed polyethylene glycol (PEG)-based hydrogels functionalized with cell instructive multifunctional biomimetic peptides (either with cyclic RGD-DWIVA or cyclic RGD-cyclic DWIVA) and cross-linked with matrix metalloproteinases (MMPs)-degradable sequences to enable dynamic enzymatic biodegradation and cell spreading and differentiation. The analysis of the intrinsic properties of the hydrogel revealed relevant mechanical properties, porosity, swelling and degradability to engineer hydrogels for bone tissue engineering. Moreover, the engineered hydrogels were able to promote human mesenchymal stem cells (MSCs) spreading and significantly improve their osteogenic differentiation. Thus, these novel hydrogels could be a promising candidate for applications in bone tissue engineering, such as acellular systems to be implanted and regenerate bone or in stem cells therapy.
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spelling pubmed-102673932023-06-15 Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering Oliver-Cervelló, Lluís Martin-Gómez, Helena Gonzalez-Garcia, Cristina Salmeron-Sanchez, Manuel Ginebra, Maria-Pau Mas-Moruno, Carlos Front Bioeng Biotechnol Bioengineering and Biotechnology Mimicking bone extracellular matrix (ECM) is paramount to develop novel biomaterials for bone tissue engineering. In this regard, the combination of integrin-binding ligands together with osteogenic peptides represents a powerful approach to recapitulate the healing microenvironment of bone. In the present work, we designed polyethylene glycol (PEG)-based hydrogels functionalized with cell instructive multifunctional biomimetic peptides (either with cyclic RGD-DWIVA or cyclic RGD-cyclic DWIVA) and cross-linked with matrix metalloproteinases (MMPs)-degradable sequences to enable dynamic enzymatic biodegradation and cell spreading and differentiation. The analysis of the intrinsic properties of the hydrogel revealed relevant mechanical properties, porosity, swelling and degradability to engineer hydrogels for bone tissue engineering. Moreover, the engineered hydrogels were able to promote human mesenchymal stem cells (MSCs) spreading and significantly improve their osteogenic differentiation. Thus, these novel hydrogels could be a promising candidate for applications in bone tissue engineering, such as acellular systems to be implanted and regenerate bone or in stem cells therapy. Frontiers Media S.A. 2023-06-01 /pmc/articles/PMC10267393/ /pubmed/37324414 http://dx.doi.org/10.3389/fbioe.2023.1192436 Text en Copyright © 2023 Oliver-Cervelló, Martin-Gómez, Gonzalez-Garcia, Salmeron-Sanchez, Ginebra and Mas-Moruno. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Oliver-Cervelló, Lluís
Martin-Gómez, Helena
Gonzalez-Garcia, Cristina
Salmeron-Sanchez, Manuel
Ginebra, Maria-Pau
Mas-Moruno, Carlos
Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title_full Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title_fullStr Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title_full_unstemmed Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title_short Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
title_sort protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267393/
https://www.ncbi.nlm.nih.gov/pubmed/37324414
http://dx.doi.org/10.3389/fbioe.2023.1192436
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