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Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma
Fluorescence-guided surgery is set to play a pivotal role in the intraoperative management of pediatric tumors. Shortwave infrared imaging (SWIR) has advantages over conventional near-infrared I (NIR-I) imaging with reduced tissue scattering and autofluorescence. Here, two NIR-I dyes (IRDye800CW and...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for Cancer Research
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267675/ https://www.ncbi.nlm.nih.gov/pubmed/36934744 http://dx.doi.org/10.1158/0008-5472.CAN-22-2918 |
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author | Privitera, Laura Waterhouse, Dale J. Preziosi, Alessandra Paraboschi, Irene Ogunlade, Olumide Da Pieve, Chiara Barisa, Marta Ogunbiyi, Olumide Weitsman, Gregory Hutchinson, J. Ciaran Cross, Kate Biassoni, Lorenzo Stoyanov, Danail Sebire, Neil Beard, Paul De Coppi, Paolo Kramer-Marek, Gabriela Anderson, John Giuliani, Stefano |
author_facet | Privitera, Laura Waterhouse, Dale J. Preziosi, Alessandra Paraboschi, Irene Ogunlade, Olumide Da Pieve, Chiara Barisa, Marta Ogunbiyi, Olumide Weitsman, Gregory Hutchinson, J. Ciaran Cross, Kate Biassoni, Lorenzo Stoyanov, Danail Sebire, Neil Beard, Paul De Coppi, Paolo Kramer-Marek, Gabriela Anderson, John Giuliani, Stefano |
author_sort | Privitera, Laura |
collection | PubMed |
description | Fluorescence-guided surgery is set to play a pivotal role in the intraoperative management of pediatric tumors. Shortwave infrared imaging (SWIR) has advantages over conventional near-infrared I (NIR-I) imaging with reduced tissue scattering and autofluorescence. Here, two NIR-I dyes (IRDye800CW and IR12), with long tails emitting in the SWIR range, were conjugated with a clinical-grade anti-GD2 monoclonal antibody (dinutuximab-beta) to compare NIR-I and SWIR imaging for neuroblastoma surgery. A first-of-its-kind multispectral NIR-I/SWIR fluorescence imaging device was constructed to allow an objective comparison between the two imaging windows. Conjugates were first characterized in vitro. Tissue-mimicking phantoms, imaging specimens of known geometric and material composition, were used to assess the sensitivity and depth penetration of the NIR-I/SWIR device, showing a minimum detectable volume of ∼0.9 mm(3) and depth penetration up to 3 mm. In vivo, fluorescence imaging using the NIR-I/SWIR device showed a high tumor-to-background ratio (TBR) for both dyes, with anti–GD2-IR800 being significantly brighter than anti–GD2-IR12. Crucially, the system enabled higher TBR at SWIR wavelengths than at NIR-I wavelengths, verifying SWIR imaging enables high-contrast delineation of tumor margins. This work demonstrates that by combining the high specificity of anti-GD2 antibodies with the availability and translatability of existing NIR-I dyes, along with the advantages of SWIR in terms of depth and tumor signal-to-background ratio, GD2-targeted NIR-I/SWIR-guided surgery could improve the treatment of patients with neuroblastoma, warranting investigation in future clinical trials. SIGNIFICANCE: Multispectral near-infrared I/shortwave infrared fluorescence imaging is a versatile system enabling high tumor-to-background signal for safer and more complete resection of pediatric tumors during surgery. |
format | Online Article Text |
id | pubmed-10267675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for Cancer Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-102676752023-06-15 Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma Privitera, Laura Waterhouse, Dale J. Preziosi, Alessandra Paraboschi, Irene Ogunlade, Olumide Da Pieve, Chiara Barisa, Marta Ogunbiyi, Olumide Weitsman, Gregory Hutchinson, J. Ciaran Cross, Kate Biassoni, Lorenzo Stoyanov, Danail Sebire, Neil Beard, Paul De Coppi, Paolo Kramer-Marek, Gabriela Anderson, John Giuliani, Stefano Cancer Res Computational Cancer Biology and Technology Fluorescence-guided surgery is set to play a pivotal role in the intraoperative management of pediatric tumors. Shortwave infrared imaging (SWIR) has advantages over conventional near-infrared I (NIR-I) imaging with reduced tissue scattering and autofluorescence. Here, two NIR-I dyes (IRDye800CW and IR12), with long tails emitting in the SWIR range, were conjugated with a clinical-grade anti-GD2 monoclonal antibody (dinutuximab-beta) to compare NIR-I and SWIR imaging for neuroblastoma surgery. A first-of-its-kind multispectral NIR-I/SWIR fluorescence imaging device was constructed to allow an objective comparison between the two imaging windows. Conjugates were first characterized in vitro. Tissue-mimicking phantoms, imaging specimens of known geometric and material composition, were used to assess the sensitivity and depth penetration of the NIR-I/SWIR device, showing a minimum detectable volume of ∼0.9 mm(3) and depth penetration up to 3 mm. In vivo, fluorescence imaging using the NIR-I/SWIR device showed a high tumor-to-background ratio (TBR) for both dyes, with anti–GD2-IR800 being significantly brighter than anti–GD2-IR12. Crucially, the system enabled higher TBR at SWIR wavelengths than at NIR-I wavelengths, verifying SWIR imaging enables high-contrast delineation of tumor margins. This work demonstrates that by combining the high specificity of anti-GD2 antibodies with the availability and translatability of existing NIR-I dyes, along with the advantages of SWIR in terms of depth and tumor signal-to-background ratio, GD2-targeted NIR-I/SWIR-guided surgery could improve the treatment of patients with neuroblastoma, warranting investigation in future clinical trials. SIGNIFICANCE: Multispectral near-infrared I/shortwave infrared fluorescence imaging is a versatile system enabling high tumor-to-background signal for safer and more complete resection of pediatric tumors during surgery. American Association for Cancer Research 2023-06-15 2023-03-19 /pmc/articles/PMC10267675/ /pubmed/36934744 http://dx.doi.org/10.1158/0008-5472.CAN-22-2918 Text en ©2023 The Authors; Published by the American Association for Cancer Research https://creativecommons.org/licenses/by/4.0/This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. |
spellingShingle | Computational Cancer Biology and Technology Privitera, Laura Waterhouse, Dale J. Preziosi, Alessandra Paraboschi, Irene Ogunlade, Olumide Da Pieve, Chiara Barisa, Marta Ogunbiyi, Olumide Weitsman, Gregory Hutchinson, J. Ciaran Cross, Kate Biassoni, Lorenzo Stoyanov, Danail Sebire, Neil Beard, Paul De Coppi, Paolo Kramer-Marek, Gabriela Anderson, John Giuliani, Stefano Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title | Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title_full | Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title_fullStr | Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title_full_unstemmed | Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title_short | Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma |
title_sort | shortwave infrared imaging enables high-contrast fluorescence-guided surgery in neuroblastoma |
topic | Computational Cancer Biology and Technology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10267675/ https://www.ncbi.nlm.nih.gov/pubmed/36934744 http://dx.doi.org/10.1158/0008-5472.CAN-22-2918 |
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