Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance

We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to...

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Autores principales: Kordalewska, Milena, Cancino-Prado, Geselle, Nobrega de Almeida Júnior, João, Brasil Brandão, Igor, Tigulini de Souza Peral, Renata, Colombo, Arnaldo L., Perlin, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269051/
https://www.ncbi.nlm.nih.gov/pubmed/37222585
http://dx.doi.org/10.1128/aac.00423-23
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author Kordalewska, Milena
Cancino-Prado, Geselle
Nobrega de Almeida Júnior, João
Brasil Brandão, Igor
Tigulini de Souza Peral, Renata
Colombo, Arnaldo L.
Perlin, David S.
author_facet Kordalewska, Milena
Cancino-Prado, Geselle
Nobrega de Almeida Júnior, João
Brasil Brandão, Igor
Tigulini de Souza Peral, Renata
Colombo, Arnaldo L.
Perlin, David S.
author_sort Kordalewska, Milena
collection PubMed
description We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32×; caspofungin, >64×; micafungin, >64×).
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spelling pubmed-102690512023-06-16 Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance Kordalewska, Milena Cancino-Prado, Geselle Nobrega de Almeida Júnior, João Brasil Brandão, Igor Tigulini de Souza Peral, Renata Colombo, Arnaldo L. Perlin, David S. Antimicrob Agents Chemother Mechanisms of Resistance We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32×; caspofungin, >64×; micafungin, >64×). American Society for Microbiology 2023-05-24 /pmc/articles/PMC10269051/ /pubmed/37222585 http://dx.doi.org/10.1128/aac.00423-23 Text en Copyright © 2023 Kordalewska et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mechanisms of Resistance
Kordalewska, Milena
Cancino-Prado, Geselle
Nobrega de Almeida Júnior, João
Brasil Brandão, Igor
Tigulini de Souza Peral, Renata
Colombo, Arnaldo L.
Perlin, David S.
Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title_full Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title_fullStr Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title_full_unstemmed Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title_short Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance
title_sort novel non-hot spot modification in fks1 of candida auris confers echinocandin resistance
topic Mechanisms of Resistance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269051/
https://www.ncbi.nlm.nih.gov/pubmed/37222585
http://dx.doi.org/10.1128/aac.00423-23
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