Cargando…
Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene
We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but β-Ca...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269617/ https://www.ncbi.nlm.nih.gov/pubmed/37074170 http://dx.doi.org/10.1128/spectrum.04620-22 |
_version_ | 1785059209440657408 |
---|---|
author | Blanco-Martín, T. Guzmán-Puche, J. Riazzo, C. Gasca-Santiyán, M. Hernández-García, M. Cantón, R. Torre-Cisneros, J. Herrera, C. Martínez-Martínez, L. |
author_facet | Blanco-Martín, T. Guzmán-Puche, J. Riazzo, C. Gasca-Santiyán, M. Hernández-García, M. Cantón, R. Torre-Cisneros, J. Herrera, C. Martínez-Martínez, L. |
author_sort | Blanco-Martín, T. |
collection | PubMed |
description | We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but β-Carba was negative. Whole-genome sequencing identified the bla(IMI-6) gene located in a conjugative IncFIIY plasmid and associated with the LysR-like regulator imiR. Both genes were bracketed by an ISEclI-like insertion sequence and a putatively defective ISEc36 insertion sequence. IMPORTANCE IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include bla(IMI) genes, which could contribute to the hidden dissemination of bacteria producing these enzymes. Techniques should be implemented to detect minor carbapenemases that are not very frequent in our environment and control their dissemination. |
format | Online Article Text |
id | pubmed-10269617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-102696172023-06-16 Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene Blanco-Martín, T. Guzmán-Puche, J. Riazzo, C. Gasca-Santiyán, M. Hernández-García, M. Cantón, R. Torre-Cisneros, J. Herrera, C. Martínez-Martínez, L. Microbiol Spectr Observation We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but β-Carba was negative. Whole-genome sequencing identified the bla(IMI-6) gene located in a conjugative IncFIIY plasmid and associated with the LysR-like regulator imiR. Both genes were bracketed by an ISEclI-like insertion sequence and a putatively defective ISEc36 insertion sequence. IMPORTANCE IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include bla(IMI) genes, which could contribute to the hidden dissemination of bacteria producing these enzymes. Techniques should be implemented to detect minor carbapenemases that are not very frequent in our environment and control their dissemination. American Society for Microbiology 2023-04-19 /pmc/articles/PMC10269617/ /pubmed/37074170 http://dx.doi.org/10.1128/spectrum.04620-22 Text en Copyright © 2023 Blanco-Martín et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Observation Blanco-Martín, T. Guzmán-Puche, J. Riazzo, C. Gasca-Santiyán, M. Hernández-García, M. Cantón, R. Torre-Cisneros, J. Herrera, C. Martínez-Martínez, L. Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title_full | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title_fullStr | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title_full_unstemmed | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title_short | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated bla(IMI-6) Gene |
title_sort | phenotypic and molecular characterization of an enterobacter ludwigii clinical isolate carrying a plasmid-mediated bla(imi-6) gene |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269617/ https://www.ncbi.nlm.nih.gov/pubmed/37074170 http://dx.doi.org/10.1128/spectrum.04620-22 |
work_keys_str_mv | AT blancomartint phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT guzmanpuchej phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT riazzoc phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT gascasantiyanm phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT hernandezgarciam phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT cantonr phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT torrecisnerosj phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT herrerac phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene AT martinezmartinezl phenotypicandmolecularcharacterizationofanenterobacterludwigiiclinicalisolatecarryingaplasmidmediatedblaimi6gene |