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Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria
Multiple millennia of human evolution have shaped the chemical composition of breast milk toward an optimal human body fluid for nutrition and protection and for shaping the early gut microbiota of newborns. This biological fluid is composed of water, lipids, simple and complex carbohydrates, protei...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269646/ https://www.ncbi.nlm.nih.gov/pubmed/37191543 http://dx.doi.org/10.1128/spectrum.00665-23 |
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author | Rizzo, Sonia Mirjam Alessandri, Giulia Lugli, Gabriele Andrea Fontana, Federico Tarracchini, Chiara Mancabelli, Leonardo Viappiani, Alice Bianchi, Massimiliano G. Bussolati, Ovidio van Sinderen, Douwe Ventura, Marco Turroni, Francesca |
author_facet | Rizzo, Sonia Mirjam Alessandri, Giulia Lugli, Gabriele Andrea Fontana, Federico Tarracchini, Chiara Mancabelli, Leonardo Viappiani, Alice Bianchi, Massimiliano G. Bussolati, Ovidio van Sinderen, Douwe Ventura, Marco Turroni, Francesca |
author_sort | Rizzo, Sonia Mirjam |
collection | PubMed |
description | Multiple millennia of human evolution have shaped the chemical composition of breast milk toward an optimal human body fluid for nutrition and protection and for shaping the early gut microbiota of newborns. This biological fluid is composed of water, lipids, simple and complex carbohydrates, proteins, immunoglobulins, and hormones. Potential interactions between hormones present in mother’s milk and the microbial community of the newborn are a very fascinating yet unexplored topic. In this context, insulin, in addition to being one of the most prevalent hormones in breast milk, is also involved in a metabolic disease that affects many pregnant women, i.e., gestational diabetes mellitus (GDM). Analysis of 3,620 publicly available metagenomic data sets revealed that the bifidobacterial community varies in relation to the different concentrations of this hormone in breast milk of healthy and diabetic mothers. Starting from this assumption, in this study, we explored possible molecular interactions between this hormone and bifidobacterial strains that represent bifidobacterial species commonly occurring in the infant gut using ‘omics’ approaches. Our findings revealed that insulin modulates the bifidobacterial community by apparently improving the persistence of the Bifidobacterium bifidum taxon in the infant gut environment compared to other typical infant-associated bifidobacterial species. IMPORTANCE Breast milk is a key factor in modulating the infant's intestinal microbiota composition. Even though the interaction between human milk sugars and bifidobacteria has been extensively studied, there are other bioactive compounds in human milk that may influence the gut microbiota, such as hormones. In this article, the molecular interaction of the human milk hormone insulin and the bifidobacterial communities colonizing the human gut in the early stages of life has been explored. This molecular cross talk was assessed using an in vitro gut microbiota model and then analyzed by various omics approaches, allowing the identification of genes associated with bacterial cell adaptation/colonization in the human intestine. Our findings provide insights into the manner by which assembly of the early gut microbiota may be regulated by host factors such as hormones carried by human milk. |
format | Online Article Text |
id | pubmed-10269646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-102696462023-06-16 Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria Rizzo, Sonia Mirjam Alessandri, Giulia Lugli, Gabriele Andrea Fontana, Federico Tarracchini, Chiara Mancabelli, Leonardo Viappiani, Alice Bianchi, Massimiliano G. Bussolati, Ovidio van Sinderen, Douwe Ventura, Marco Turroni, Francesca Microbiol Spectr Research Article Multiple millennia of human evolution have shaped the chemical composition of breast milk toward an optimal human body fluid for nutrition and protection and for shaping the early gut microbiota of newborns. This biological fluid is composed of water, lipids, simple and complex carbohydrates, proteins, immunoglobulins, and hormones. Potential interactions between hormones present in mother’s milk and the microbial community of the newborn are a very fascinating yet unexplored topic. In this context, insulin, in addition to being one of the most prevalent hormones in breast milk, is also involved in a metabolic disease that affects many pregnant women, i.e., gestational diabetes mellitus (GDM). Analysis of 3,620 publicly available metagenomic data sets revealed that the bifidobacterial community varies in relation to the different concentrations of this hormone in breast milk of healthy and diabetic mothers. Starting from this assumption, in this study, we explored possible molecular interactions between this hormone and bifidobacterial strains that represent bifidobacterial species commonly occurring in the infant gut using ‘omics’ approaches. Our findings revealed that insulin modulates the bifidobacterial community by apparently improving the persistence of the Bifidobacterium bifidum taxon in the infant gut environment compared to other typical infant-associated bifidobacterial species. IMPORTANCE Breast milk is a key factor in modulating the infant's intestinal microbiota composition. Even though the interaction between human milk sugars and bifidobacteria has been extensively studied, there are other bioactive compounds in human milk that may influence the gut microbiota, such as hormones. In this article, the molecular interaction of the human milk hormone insulin and the bifidobacterial communities colonizing the human gut in the early stages of life has been explored. This molecular cross talk was assessed using an in vitro gut microbiota model and then analyzed by various omics approaches, allowing the identification of genes associated with bacterial cell adaptation/colonization in the human intestine. Our findings provide insights into the manner by which assembly of the early gut microbiota may be regulated by host factors such as hormones carried by human milk. American Society for Microbiology 2023-05-16 /pmc/articles/PMC10269646/ /pubmed/37191543 http://dx.doi.org/10.1128/spectrum.00665-23 Text en Copyright © 2023 Rizzo et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Rizzo, Sonia Mirjam Alessandri, Giulia Lugli, Gabriele Andrea Fontana, Federico Tarracchini, Chiara Mancabelli, Leonardo Viappiani, Alice Bianchi, Massimiliano G. Bussolati, Ovidio van Sinderen, Douwe Ventura, Marco Turroni, Francesca Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title | Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title_full | Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title_fullStr | Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title_full_unstemmed | Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title_short | Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria |
title_sort | exploring molecular interactions between human milk hormone insulin and bifidobacteria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269646/ https://www.ncbi.nlm.nih.gov/pubmed/37191543 http://dx.doi.org/10.1128/spectrum.00665-23 |
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