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Targeting the 16S rRNA Gene by Reverse Complement PCR Next-Generation Sequencing: Specific and Sensitive Detection and Identification of Microbes Directly in Clinical Samples

The detection and accurate identification of bacterial species in clinical samples are crucial for diagnosis and appropriate antibiotic treatment. To date, sequencing of the 16S rRNA gene has been widely used as a complementary molecular approach when identification by culture fails. The accuracy an...

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Detalles Bibliográficos
Autores principales: Moorlag, Simone J. C. F. M., Coolen, Jordy P. M., van den Bosch, Bart, Jin, Elisabeth Hui-Mei, Buil, Jochem B., Wertheim, Heiman F. L., Melchers, Willem J. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269728/
https://www.ncbi.nlm.nih.gov/pubmed/37227289
http://dx.doi.org/10.1128/spectrum.04483-22

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