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Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood

PCR can be a supplement to Treponema serological testing. However, its sensitivity is not satisfactory for blood sample testing. The aim of this study was to investigate whether pretreatment with red blood cell (RBC) lysis could enhance the yield of Treponema pallidum subsp. pallidum DNA extraction...

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Detalles Bibliográficos
Autores principales: Wei, Zi-Han, Xie, Lin, Wang, Yong-Jing, Zhuang, Jiang-Xing, Niu, Jian-Jun, Liu, Li-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10269841/
https://www.ncbi.nlm.nih.gov/pubmed/37222624
http://dx.doi.org/10.1128/spectrum.05198-22