Cargando…
RNA granules: functional compartments or incidental condensates?
RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270194/ https://www.ncbi.nlm.nih.gov/pubmed/37137715 http://dx.doi.org/10.1101/gad.350518.123 |
_version_ | 1785059289378848768 |
---|---|
author | Putnam, Andrea Thomas, Laura Seydoux, Geraldine |
author_facet | Putnam, Andrea Thomas, Laura Seydoux, Geraldine |
author_sort | Putnam, Andrea |
collection | PubMed |
description | RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation of subsoluble ribonucleoprotein (RNP) complexes that partially demix from the cytoplasm or nucleoplasm. We explore the possibility that some RNA granules are nonessential condensation by-products that arise when RNP complexes exceed their solubility limit as a consequence of cellular activity, stress, or aging. We describe the use of evolutionary and mutational analyses and single-molecule techniques to distinguish functional RNA granules from “incidental condensates.” |
format | Online Article Text |
id | pubmed-10270194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102701942023-06-16 RNA granules: functional compartments or incidental condensates? Putnam, Andrea Thomas, Laura Seydoux, Geraldine Genes Dev Review RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation of subsoluble ribonucleoprotein (RNP) complexes that partially demix from the cytoplasm or nucleoplasm. We explore the possibility that some RNA granules are nonessential condensation by-products that arise when RNP complexes exceed their solubility limit as a consequence of cellular activity, stress, or aging. We describe the use of evolutionary and mutational analyses and single-molecule techniques to distinguish functional RNA granules from “incidental condensates.” Cold Spring Harbor Laboratory Press 2023-05-01 /pmc/articles/PMC10270194/ /pubmed/37137715 http://dx.doi.org/10.1101/gad.350518.123 Text en © 2023 Putnam et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by/4.0/This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Putnam, Andrea Thomas, Laura Seydoux, Geraldine RNA granules: functional compartments or incidental condensates? |
title | RNA granules: functional compartments or incidental condensates? |
title_full | RNA granules: functional compartments or incidental condensates? |
title_fullStr | RNA granules: functional compartments or incidental condensates? |
title_full_unstemmed | RNA granules: functional compartments or incidental condensates? |
title_short | RNA granules: functional compartments or incidental condensates? |
title_sort | rna granules: functional compartments or incidental condensates? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270194/ https://www.ncbi.nlm.nih.gov/pubmed/37137715 http://dx.doi.org/10.1101/gad.350518.123 |
work_keys_str_mv | AT putnamandrea rnagranulesfunctionalcompartmentsorincidentalcondensates AT thomaslaura rnagranulesfunctionalcompartmentsorincidentalcondensates AT seydouxgeraldine rnagranulesfunctionalcompartmentsorincidentalcondensates |