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RNA granules: functional compartments or incidental condensates?

RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation...

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Detalles Bibliográficos
Autores principales: Putnam, Andrea, Thomas, Laura, Seydoux, Geraldine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270194/
https://www.ncbi.nlm.nih.gov/pubmed/37137715
http://dx.doi.org/10.1101/gad.350518.123
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author Putnam, Andrea
Thomas, Laura
Seydoux, Geraldine
author_facet Putnam, Andrea
Thomas, Laura
Seydoux, Geraldine
author_sort Putnam, Andrea
collection PubMed
description RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation of subsoluble ribonucleoprotein (RNP) complexes that partially demix from the cytoplasm or nucleoplasm. We explore the possibility that some RNA granules are nonessential condensation by-products that arise when RNP complexes exceed their solubility limit as a consequence of cellular activity, stress, or aging. We describe the use of evolutionary and mutational analyses and single-molecule techniques to distinguish functional RNA granules from “incidental condensates.”
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spelling pubmed-102701942023-06-16 RNA granules: functional compartments or incidental condensates? Putnam, Andrea Thomas, Laura Seydoux, Geraldine Genes Dev Review RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation of subsoluble ribonucleoprotein (RNP) complexes that partially demix from the cytoplasm or nucleoplasm. We explore the possibility that some RNA granules are nonessential condensation by-products that arise when RNP complexes exceed their solubility limit as a consequence of cellular activity, stress, or aging. We describe the use of evolutionary and mutational analyses and single-molecule techniques to distinguish functional RNA granules from “incidental condensates.” Cold Spring Harbor Laboratory Press 2023-05-01 /pmc/articles/PMC10270194/ /pubmed/37137715 http://dx.doi.org/10.1101/gad.350518.123 Text en © 2023 Putnam et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by/4.0/This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Putnam, Andrea
Thomas, Laura
Seydoux, Geraldine
RNA granules: functional compartments or incidental condensates?
title RNA granules: functional compartments or incidental condensates?
title_full RNA granules: functional compartments or incidental condensates?
title_fullStr RNA granules: functional compartments or incidental condensates?
title_full_unstemmed RNA granules: functional compartments or incidental condensates?
title_short RNA granules: functional compartments or incidental condensates?
title_sort rna granules: functional compartments or incidental condensates?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270194/
https://www.ncbi.nlm.nih.gov/pubmed/37137715
http://dx.doi.org/10.1101/gad.350518.123
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