Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the constructi...

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Detalles Bibliográficos
Autores principales: Meng, Fan, Guo, Jiao, Wang, Shan, Zhang, Changhui, Li, Xuehui, Chen, Yanjuan, Shi, Qiuli, Shao, Liang, Zhang, Rundong, Wang, Ganggang, Su, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270729/
https://www.ncbi.nlm.nih.gov/pubmed/37342585
http://dx.doi.org/10.1016/j.heliyon.2023.e17364
Descripción
Sumario:The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research.

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