Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the constructi...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Fan, Guo, Jiao, Wang, Shan, Zhang, Changhui, Li, Xuehui, Chen, Yanjuan, Shi, Qiuli, Shao, Liang, Zhang, Rundong, Wang, Ganggang, Su, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270729/
https://www.ncbi.nlm.nih.gov/pubmed/37342585
http://dx.doi.org/10.1016/j.heliyon.2023.e17364
_version_ 1785059376725229568
author Meng, Fan
Guo, Jiao
Wang, Shan
Zhang, Changhui
Li, Xuehui
Chen, Yanjuan
Shi, Qiuli
Shao, Liang
Zhang, Rundong
Wang, Ganggang
Su, Dan
author_facet Meng, Fan
Guo, Jiao
Wang, Shan
Zhang, Changhui
Li, Xuehui
Chen, Yanjuan
Shi, Qiuli
Shao, Liang
Zhang, Rundong
Wang, Ganggang
Su, Dan
author_sort Meng, Fan
collection PubMed
description The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research.
format Online
Article
Text
id pubmed-10270729
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-102707292023-06-16 Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method Meng, Fan Guo, Jiao Wang, Shan Zhang, Changhui Li, Xuehui Chen, Yanjuan Shi, Qiuli Shao, Liang Zhang, Rundong Wang, Ganggang Su, Dan Heliyon Research Article The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research. Elsevier 2023-06-16 /pmc/articles/PMC10270729/ /pubmed/37342585 http://dx.doi.org/10.1016/j.heliyon.2023.e17364 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Meng, Fan
Guo, Jiao
Wang, Shan
Zhang, Changhui
Li, Xuehui
Chen, Yanjuan
Shi, Qiuli
Shao, Liang
Zhang, Rundong
Wang, Ganggang
Su, Dan
Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title_full Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title_fullStr Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title_full_unstemmed Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title_short Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
title_sort fast construction of sars-cov-2 associated plasmid library using parallel cloning method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270729/
https://www.ncbi.nlm.nih.gov/pubmed/37342585
http://dx.doi.org/10.1016/j.heliyon.2023.e17364
work_keys_str_mv AT mengfan fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT guojiao fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT wangshan fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT zhangchanghui fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT lixuehui fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT chenyanjuan fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT shiqiuli fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT shaoliang fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT zhangrundong fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT wangganggang fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod
AT sudan fastconstructionofsarscov2associatedplasmidlibraryusingparallelcloningmethod

Ejemplares similares