Cargando…

Bismuth release from endodontic materials: in vivo analysis using Wistar rats

Calcium silicate-based materials are used to block the communication between the root canal and the periodontal ligament space. This brings the materials into contact with tissues and the potential for local and systemic elemental release and movement. The aim of the study was to evaluate the elemen...

Descripción completa

Detalles Bibliográficos
Autores principales: Marciano, M. A., Pelepenko, L. E., Francati, T. M., Antunes, T. B. M., Janini, A. C. P., Rohwedder, J. J. R., Shelton, R. M., Camilleri, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272127/
https://www.ncbi.nlm.nih.gov/pubmed/37322106
http://dx.doi.org/10.1038/s41598-023-36690-4
_version_ 1785059424192167936
author Marciano, M. A.
Pelepenko, L. E.
Francati, T. M.
Antunes, T. B. M.
Janini, A. C. P.
Rohwedder, J. J. R.
Shelton, R. M.
Camilleri, J.
author_facet Marciano, M. A.
Pelepenko, L. E.
Francati, T. M.
Antunes, T. B. M.
Janini, A. C. P.
Rohwedder, J. J. R.
Shelton, R. M.
Camilleri, J.
author_sort Marciano, M. A.
collection PubMed
description Calcium silicate-based materials are used to block the communication between the root canal and the periodontal ligament space. This brings the materials into contact with tissues and the potential for local and systemic elemental release and movement. The aim of the study was to evaluate the elemental release of bismuth from ProRoot MTA in contact with connective tissues after 30 and 180 days as well as any accumulation in peripheral organs using an animal model. Tricalcium silicate and hydroxyapatite containing 20% bismuth oxide (HAp-Bi) were used as controls. The null hypothesis was that bismuth migrates from tricalcium silicate-based materials when associated with silicon. The materials were examined using scanning electron microscopy, energy dispersive spectroscopy (SEM/EDS) and X-ray diffraction prior to implantation as well as using SEM/EDS, micro X-ray fluorescence and Raman spectroscopy after implantation to assess elemental presence in surrounding tissues. Histological analysis was used to evaluate the changes in tissue architecture and inductively coupled plasma mass spectrometry (ICP-MS) was used to investigate the elemental deposition. For the systemic investigation, routine blood analysis was performed and organs were obtained to evaluate the presence of bismuth and silicon using ICP-MS after acid digestion. In the histological analysis of the implantation sites, macrophages and multinucleated giant cells could be observed after 30 days which after 180 days became a chronic infiltrate; although, no major differences were identified in red and white blood cell analyses and biochemical tests. Implantation altered the materials as observed in the Raman analysis and bismuth was detected both locally and within kidney samples after both periods of analysis, indicating the potential for accumulation of bismuth in this organ. Smaller amounts of bismuth than observed in the kidney were also detected in blood, liver and brain for the ProRoot MTA and HAp-Bi after 180 days. Bismuth was released from the ProRoot MTA locally and was detected systemically and in samples without silicon; thus, the null hypothesis was rejected. The bismuth release demonstrated that this element accumulated both locally and systemically, mainly in the kidneys in comparison with brain and liver regardless of the material base.
format Online
Article
Text
id pubmed-10272127
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-102721272023-06-17 Bismuth release from endodontic materials: in vivo analysis using Wistar rats Marciano, M. A. Pelepenko, L. E. Francati, T. M. Antunes, T. B. M. Janini, A. C. P. Rohwedder, J. J. R. Shelton, R. M. Camilleri, J. Sci Rep Article Calcium silicate-based materials are used to block the communication between the root canal and the periodontal ligament space. This brings the materials into contact with tissues and the potential for local and systemic elemental release and movement. The aim of the study was to evaluate the elemental release of bismuth from ProRoot MTA in contact with connective tissues after 30 and 180 days as well as any accumulation in peripheral organs using an animal model. Tricalcium silicate and hydroxyapatite containing 20% bismuth oxide (HAp-Bi) were used as controls. The null hypothesis was that bismuth migrates from tricalcium silicate-based materials when associated with silicon. The materials were examined using scanning electron microscopy, energy dispersive spectroscopy (SEM/EDS) and X-ray diffraction prior to implantation as well as using SEM/EDS, micro X-ray fluorescence and Raman spectroscopy after implantation to assess elemental presence in surrounding tissues. Histological analysis was used to evaluate the changes in tissue architecture and inductively coupled plasma mass spectrometry (ICP-MS) was used to investigate the elemental deposition. For the systemic investigation, routine blood analysis was performed and organs were obtained to evaluate the presence of bismuth and silicon using ICP-MS after acid digestion. In the histological analysis of the implantation sites, macrophages and multinucleated giant cells could be observed after 30 days which after 180 days became a chronic infiltrate; although, no major differences were identified in red and white blood cell analyses and biochemical tests. Implantation altered the materials as observed in the Raman analysis and bismuth was detected both locally and within kidney samples after both periods of analysis, indicating the potential for accumulation of bismuth in this organ. Smaller amounts of bismuth than observed in the kidney were also detected in blood, liver and brain for the ProRoot MTA and HAp-Bi after 180 days. Bismuth was released from the ProRoot MTA locally and was detected systemically and in samples without silicon; thus, the null hypothesis was rejected. The bismuth release demonstrated that this element accumulated both locally and systemically, mainly in the kidneys in comparison with brain and liver regardless of the material base. Nature Publishing Group UK 2023-06-15 /pmc/articles/PMC10272127/ /pubmed/37322106 http://dx.doi.org/10.1038/s41598-023-36690-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Marciano, M. A.
Pelepenko, L. E.
Francati, T. M.
Antunes, T. B. M.
Janini, A. C. P.
Rohwedder, J. J. R.
Shelton, R. M.
Camilleri, J.
Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title_full Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title_fullStr Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title_full_unstemmed Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title_short Bismuth release from endodontic materials: in vivo analysis using Wistar rats
title_sort bismuth release from endodontic materials: in vivo analysis using wistar rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272127/
https://www.ncbi.nlm.nih.gov/pubmed/37322106
http://dx.doi.org/10.1038/s41598-023-36690-4
work_keys_str_mv AT marcianoma bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT pelepenkole bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT francatitm bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT antunestbm bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT janiniacp bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT rohwedderjjr bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT sheltonrm bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats
AT camillerij bismuthreleasefromendodonticmaterialsinvivoanalysisusingwistarrats