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Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways

WRKY transcription factors (TFs) play an important role in regulating the mechanism of plant self-defense. However, the function of most WRKY TFs in upland cotton (Gossypium hirsutum) is still unknown. Hence, studying the molecular mechanism of WRKY TFs in the resistance of cotton to Verticillium da...

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Autores principales: Li, Youzhong, Chen, Haihong, Wang, Youwu, Zhu, Jincheng, Zhang, Xiaoli, Sun, Jie, Liu, Feng, Zhao, Yiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272532/
https://www.ncbi.nlm.nih.gov/pubmed/37332701
http://dx.doi.org/10.3389/fpls.2023.1203695
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author Li, Youzhong
Chen, Haihong
Wang, Youwu
Zhu, Jincheng
Zhang, Xiaoli
Sun, Jie
Liu, Feng
Zhao, Yiying
author_facet Li, Youzhong
Chen, Haihong
Wang, Youwu
Zhu, Jincheng
Zhang, Xiaoli
Sun, Jie
Liu, Feng
Zhao, Yiying
author_sort Li, Youzhong
collection PubMed
description WRKY transcription factors (TFs) play an important role in regulating the mechanism of plant self-defense. However, the function of most WRKY TFs in upland cotton (Gossypium hirsutum) is still unknown. Hence, studying the molecular mechanism of WRKY TFs in the resistance of cotton to Verticillium dahliae is of great significance to enhancing cotton disease resistance and improving its fiber quality. In this study, Bioinformatics has been used to characterize the cotton WRKY53 gene family. we analyzed the GhWRKY53 expression patterns in different resistant upland cotton cultivars treated with salicylic acid (SA) and methyl jasmonate (MeJA). Additionally, GhWRKY53 was silenced using a virus-induced gene silencing (VIGS) to determine the contribution of GhWRKY53 to V. dahliae resistance in cotton. The result showed that GhWRKY53 mediated SA and MeJA signal transduction pathways. After VIGS of the GhWRKY53, the ability of cotton to resist V. dahliae decreased, indicating that the GhWRKY53 could be involved in the disease resistance mechanism of cotton. Studies on the levels of SA and jasmonic acid (JA) and their related pathway genes demonstrated that the silencing of GhWRKY53 inhibited the SA pathway and activated the JA pathway, thereby reducing the resistance of plants to V. dahliae. In conclusion, GhWRKY53 could change the tolerance of upland cotton to V. dahliae by regulating the expression of SA and JA pathway-related genes. However, the interaction mechanism between JA and SA signaling pathways in cotton in response to V. dahliae requires further study.
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spelling pubmed-102725322023-06-17 Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways Li, Youzhong Chen, Haihong Wang, Youwu Zhu, Jincheng Zhang, Xiaoli Sun, Jie Liu, Feng Zhao, Yiying Front Plant Sci Plant Science WRKY transcription factors (TFs) play an important role in regulating the mechanism of plant self-defense. However, the function of most WRKY TFs in upland cotton (Gossypium hirsutum) is still unknown. Hence, studying the molecular mechanism of WRKY TFs in the resistance of cotton to Verticillium dahliae is of great significance to enhancing cotton disease resistance and improving its fiber quality. In this study, Bioinformatics has been used to characterize the cotton WRKY53 gene family. we analyzed the GhWRKY53 expression patterns in different resistant upland cotton cultivars treated with salicylic acid (SA) and methyl jasmonate (MeJA). Additionally, GhWRKY53 was silenced using a virus-induced gene silencing (VIGS) to determine the contribution of GhWRKY53 to V. dahliae resistance in cotton. The result showed that GhWRKY53 mediated SA and MeJA signal transduction pathways. After VIGS of the GhWRKY53, the ability of cotton to resist V. dahliae decreased, indicating that the GhWRKY53 could be involved in the disease resistance mechanism of cotton. Studies on the levels of SA and jasmonic acid (JA) and their related pathway genes demonstrated that the silencing of GhWRKY53 inhibited the SA pathway and activated the JA pathway, thereby reducing the resistance of plants to V. dahliae. In conclusion, GhWRKY53 could change the tolerance of upland cotton to V. dahliae by regulating the expression of SA and JA pathway-related genes. However, the interaction mechanism between JA and SA signaling pathways in cotton in response to V. dahliae requires further study. Frontiers Media S.A. 2023-06-02 /pmc/articles/PMC10272532/ /pubmed/37332701 http://dx.doi.org/10.3389/fpls.2023.1203695 Text en Copyright © 2023 Li, Chen, Wang, Zhu, Zhang, Sun, Liu and Zhao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Youzhong
Chen, Haihong
Wang, Youwu
Zhu, Jincheng
Zhang, Xiaoli
Sun, Jie
Liu, Feng
Zhao, Yiying
Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title_full Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title_fullStr Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title_full_unstemmed Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title_short Function analysis of GhWRKY53 regulating cotton resistance to verticillium wilt by JA and SA signaling pathways
title_sort function analysis of ghwrky53 regulating cotton resistance to verticillium wilt by ja and sa signaling pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272532/
https://www.ncbi.nlm.nih.gov/pubmed/37332701
http://dx.doi.org/10.3389/fpls.2023.1203695
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