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Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury

Epithelial regeneration is critical for barrier maintenance and organ function after intestinal radiation injury. Accumulating evidence indicates that the interleukin family members play critical roles in intestinal stem-cell-mediated epithelial regeneration. However, little is known about the relat...

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Autores principales: Guan, Ruoyu, Pan, Mengxue, Xu, Xiaoya, Du, Lixia, Rao, Xinxin, Fu, Guoxiang, Lv, Tao, Zhang, Long, Li, Yuanchuang, Tang, Peiyuan, Zhou, Yi, Wang, Yanqing, Zhang, Zhen, Gao, Jianjun, Zhou, Hong, Mi, Wenli, Hua, Guoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272662/
https://www.ncbi.nlm.nih.gov/pubmed/37291802
http://dx.doi.org/10.1177/09636897231177377
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author Guan, Ruoyu
Pan, Mengxue
Xu, Xiaoya
Du, Lixia
Rao, Xinxin
Fu, Guoxiang
Lv, Tao
Zhang, Long
Li, Yuanchuang
Tang, Peiyuan
Zhou, Yi
Wang, Yanqing
Zhang, Zhen
Gao, Jianjun
Zhou, Hong
Mi, Wenli
Hua, Guoqiang
author_facet Guan, Ruoyu
Pan, Mengxue
Xu, Xiaoya
Du, Lixia
Rao, Xinxin
Fu, Guoxiang
Lv, Tao
Zhang, Long
Li, Yuanchuang
Tang, Peiyuan
Zhou, Yi
Wang, Yanqing
Zhang, Zhen
Gao, Jianjun
Zhou, Hong
Mi, Wenli
Hua, Guoqiang
author_sort Guan, Ruoyu
collection PubMed
description Epithelial regeneration is critical for barrier maintenance and organ function after intestinal radiation injury. Accumulating evidence indicates that the interleukin family members play critical roles in intestinal stem-cell-mediated epithelial regeneration. However, little is known about the relationship between interleukin 33 (IL-33)/ST2 axis and intestinal regeneration after radiation injury. We demonstrate here that IL-33 expression significantly increased after radiation treatment. Deficiency of IL-33/ST2 promotes intestinal epithelial regeneration, resulting in a reduction of mortality during radiation-induced intestine injury. Using ex vivo organoid cultures, we show that recombinant IL-33 promotes intestinal stem cell differentiation. Mechanistically, the effects of IL-33 were mediated by activation of transforming growth factor-β signaling. Our findings reveal a fundamental mechanism by which IL-33 is able to regulate the intestinal crypt regeneration after tissue damage.
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spelling pubmed-102726622023-06-17 Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury Guan, Ruoyu Pan, Mengxue Xu, Xiaoya Du, Lixia Rao, Xinxin Fu, Guoxiang Lv, Tao Zhang, Long Li, Yuanchuang Tang, Peiyuan Zhou, Yi Wang, Yanqing Zhang, Zhen Gao, Jianjun Zhou, Hong Mi, Wenli Hua, Guoqiang Cell Transplant Original Article Epithelial regeneration is critical for barrier maintenance and organ function after intestinal radiation injury. Accumulating evidence indicates that the interleukin family members play critical roles in intestinal stem-cell-mediated epithelial regeneration. However, little is known about the relationship between interleukin 33 (IL-33)/ST2 axis and intestinal regeneration after radiation injury. We demonstrate here that IL-33 expression significantly increased after radiation treatment. Deficiency of IL-33/ST2 promotes intestinal epithelial regeneration, resulting in a reduction of mortality during radiation-induced intestine injury. Using ex vivo organoid cultures, we show that recombinant IL-33 promotes intestinal stem cell differentiation. Mechanistically, the effects of IL-33 were mediated by activation of transforming growth factor-β signaling. Our findings reveal a fundamental mechanism by which IL-33 is able to regulate the intestinal crypt regeneration after tissue damage. SAGE Publications 2023-06-08 /pmc/articles/PMC10272662/ /pubmed/37291802 http://dx.doi.org/10.1177/09636897231177377 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Guan, Ruoyu
Pan, Mengxue
Xu, Xiaoya
Du, Lixia
Rao, Xinxin
Fu, Guoxiang
Lv, Tao
Zhang, Long
Li, Yuanchuang
Tang, Peiyuan
Zhou, Yi
Wang, Yanqing
Zhang, Zhen
Gao, Jianjun
Zhou, Hong
Mi, Wenli
Hua, Guoqiang
Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title_full Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title_fullStr Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title_full_unstemmed Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title_short Interleukin-33 Potentiates TGF-β Signaling to Regulate Intestinal Stem Cell Regeneration After Radiation Injury
title_sort interleukin-33 potentiates tgf-β signaling to regulate intestinal stem cell regeneration after radiation injury
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272662/
https://www.ncbi.nlm.nih.gov/pubmed/37291802
http://dx.doi.org/10.1177/09636897231177377
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