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Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells

Previous studies transplanted human-induced pluripotent stem cells (hiPSCs)-derived mesenchymal stem cells (iMSCs) into thyroid cartilage defect of X-liked severe combined immunodeficiency (X-SCID) rats and confirmed transplanted cell survival and cartilage regeneration. Thus, this study aimed to in...

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Autores principales: Mizuno, Keisuke, Ohnishi, Hiroe, Yoshimatsu, Masayoshi, Zhao, Chengzhu, Hayashi, Yasuyuki, Kuwata, Fumihiko, Kaba, Shinji, Okuyama, Hideaki, Kawai, Yoshitaka, Hiwatashi, Nao, Kishimoto, Yo, Sakamoto, Tatsunori, Ikeya, Makoto, Omori, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272670/
https://www.ncbi.nlm.nih.gov/pubmed/37278405
http://dx.doi.org/10.1177/09636897231178460
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author Mizuno, Keisuke
Ohnishi, Hiroe
Yoshimatsu, Masayoshi
Zhao, Chengzhu
Hayashi, Yasuyuki
Kuwata, Fumihiko
Kaba, Shinji
Okuyama, Hideaki
Kawai, Yoshitaka
Hiwatashi, Nao
Kishimoto, Yo
Sakamoto, Tatsunori
Ikeya, Makoto
Omori, Koichi
author_facet Mizuno, Keisuke
Ohnishi, Hiroe
Yoshimatsu, Masayoshi
Zhao, Chengzhu
Hayashi, Yasuyuki
Kuwata, Fumihiko
Kaba, Shinji
Okuyama, Hideaki
Kawai, Yoshitaka
Hiwatashi, Nao
Kishimoto, Yo
Sakamoto, Tatsunori
Ikeya, Makoto
Omori, Koichi
author_sort Mizuno, Keisuke
collection PubMed
description Previous studies transplanted human-induced pluripotent stem cells (hiPSCs)-derived mesenchymal stem cells (iMSCs) into thyroid cartilage defect of X-liked severe combined immunodeficiency (X-SCID) rats and confirmed transplanted cell survival and cartilage regeneration. Thus, this study aimed to investigate the contribution of iMSC transplantation to thyroid cartilage regeneration of nude rats. iMSCs were induced from hiPSCs via a neural crest cell lineage. Then, clumps formed from an iMSC/extracellular matrix complex were transplanted into thyroid cartilage defects in nude rats. The larynx was removed and histological and immunohistochemical analyses were performed 4 or 8 weeks after the transplantation. Human nuclear antigen (HNA)-positive cells were observed in 11 of 12 (91.7%) rats, which indicated that transplanted iMSCs survived in thyroid cartilage defects in nude rats. HNA-positive cells co-expressed SOX9, and type II collagen was identified around HNA-positive cells in 8 of 12 rats (66.7%), which indicated cartilage-like regeneration. Cartilage-like regeneration in nude rats in this study was comparable to the previous report on X-SCID rats (HNA-positive cells were observed in all 14 rats and cartilage-like regeneration was observed in 10 of 14 rats). This result suggests that nude rats could be an alternative to X-SCID rats in thyroid cartilage regeneration experiments using iMSCs, and this nude rat cartilage transplantation model may develop cartilage regeneration research concerning fewer problems such as infection due to immunosuppression.
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spelling pubmed-102726702023-06-17 Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells Mizuno, Keisuke Ohnishi, Hiroe Yoshimatsu, Masayoshi Zhao, Chengzhu Hayashi, Yasuyuki Kuwata, Fumihiko Kaba, Shinji Okuyama, Hideaki Kawai, Yoshitaka Hiwatashi, Nao Kishimoto, Yo Sakamoto, Tatsunori Ikeya, Makoto Omori, Koichi Cell Transplant Original Article Previous studies transplanted human-induced pluripotent stem cells (hiPSCs)-derived mesenchymal stem cells (iMSCs) into thyroid cartilage defect of X-liked severe combined immunodeficiency (X-SCID) rats and confirmed transplanted cell survival and cartilage regeneration. Thus, this study aimed to investigate the contribution of iMSC transplantation to thyroid cartilage regeneration of nude rats. iMSCs were induced from hiPSCs via a neural crest cell lineage. Then, clumps formed from an iMSC/extracellular matrix complex were transplanted into thyroid cartilage defects in nude rats. The larynx was removed and histological and immunohistochemical analyses were performed 4 or 8 weeks after the transplantation. Human nuclear antigen (HNA)-positive cells were observed in 11 of 12 (91.7%) rats, which indicated that transplanted iMSCs survived in thyroid cartilage defects in nude rats. HNA-positive cells co-expressed SOX9, and type II collagen was identified around HNA-positive cells in 8 of 12 rats (66.7%), which indicated cartilage-like regeneration. Cartilage-like regeneration in nude rats in this study was comparable to the previous report on X-SCID rats (HNA-positive cells were observed in all 14 rats and cartilage-like regeneration was observed in 10 of 14 rats). This result suggests that nude rats could be an alternative to X-SCID rats in thyroid cartilage regeneration experiments using iMSCs, and this nude rat cartilage transplantation model may develop cartilage regeneration research concerning fewer problems such as infection due to immunosuppression. SAGE Publications 2023-06-06 /pmc/articles/PMC10272670/ /pubmed/37278405 http://dx.doi.org/10.1177/09636897231178460 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Mizuno, Keisuke
Ohnishi, Hiroe
Yoshimatsu, Masayoshi
Zhao, Chengzhu
Hayashi, Yasuyuki
Kuwata, Fumihiko
Kaba, Shinji
Okuyama, Hideaki
Kawai, Yoshitaka
Hiwatashi, Nao
Kishimoto, Yo
Sakamoto, Tatsunori
Ikeya, Makoto
Omori, Koichi
Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title_full Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title_fullStr Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title_full_unstemmed Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title_short Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells
title_sort laryngeal cartilage regeneration of nude rats by transplantation of mesenchymal stem cells derived from human-induced pluripotent stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272670/
https://www.ncbi.nlm.nih.gov/pubmed/37278405
http://dx.doi.org/10.1177/09636897231178460
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