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A histochemical reporter system to study extracellular ATP response in plants
When cells experience acute mechanical distress, they release ATP from their cellular compartment into the surrounding microenvironment. This extracellular ATP (eATP) can then act as a danger signal—signaling cellular damage. In plants, cells adjacent to damage detect rising eATP concentrations thro...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272726/ https://www.ncbi.nlm.nih.gov/pubmed/37332691 http://dx.doi.org/10.3389/fpls.2023.1183335 |
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author | Sowders, Joel M. Tanaka, Kiwamu |
author_facet | Sowders, Joel M. Tanaka, Kiwamu |
author_sort | Sowders, Joel M. |
collection | PubMed |
description | When cells experience acute mechanical distress, they release ATP from their cellular compartment into the surrounding microenvironment. This extracellular ATP (eATP) can then act as a danger signal—signaling cellular damage. In plants, cells adjacent to damage detect rising eATP concentrations through the cell-surface receptor kinase, P2K1. Following eATP perception, P2K1 initiates a signaling cascade mobilizing plant defense. Recent transcriptome analysis revealed a profile of eATP-induced genes sharing pathogen- and wound-response hallmarks—consistent with a working model for eATP as a defense-mobilizing danger signal. To build on the transcriptional footprint and broaden our understanding of dynamic eATP signaling responses in plants, we aimed to i) generate a visual toolkit for eATP-inducible marker genes using a β-glucuronidase (GUS) reporter system and ii) evaluate the spatiotemporal response of these genes to eATP in plant tissues. Here, we demonstrate that the promoter activities of five genes, ATPR1, ATPR2, TAT3, WRKY46, and CNGC19, were highly sensitive to eATP in the primary root meristem and elongation zones with maximal responses at 2 h after treatment. These results suggest the primary root tip as a hub to study eATP-signaling activity and provide a proof-of-concept toward using these reporters to further dissect eATP and damage signaling in plants. |
format | Online Article Text |
id | pubmed-10272726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102727262023-06-17 A histochemical reporter system to study extracellular ATP response in plants Sowders, Joel M. Tanaka, Kiwamu Front Plant Sci Plant Science When cells experience acute mechanical distress, they release ATP from their cellular compartment into the surrounding microenvironment. This extracellular ATP (eATP) can then act as a danger signal—signaling cellular damage. In plants, cells adjacent to damage detect rising eATP concentrations through the cell-surface receptor kinase, P2K1. Following eATP perception, P2K1 initiates a signaling cascade mobilizing plant defense. Recent transcriptome analysis revealed a profile of eATP-induced genes sharing pathogen- and wound-response hallmarks—consistent with a working model for eATP as a defense-mobilizing danger signal. To build on the transcriptional footprint and broaden our understanding of dynamic eATP signaling responses in plants, we aimed to i) generate a visual toolkit for eATP-inducible marker genes using a β-glucuronidase (GUS) reporter system and ii) evaluate the spatiotemporal response of these genes to eATP in plant tissues. Here, we demonstrate that the promoter activities of five genes, ATPR1, ATPR2, TAT3, WRKY46, and CNGC19, were highly sensitive to eATP in the primary root meristem and elongation zones with maximal responses at 2 h after treatment. These results suggest the primary root tip as a hub to study eATP-signaling activity and provide a proof-of-concept toward using these reporters to further dissect eATP and damage signaling in plants. Frontiers Media S.A. 2023-06-02 /pmc/articles/PMC10272726/ /pubmed/37332691 http://dx.doi.org/10.3389/fpls.2023.1183335 Text en Copyright © 2023 Sowders and Tanaka https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Sowders, Joel M. Tanaka, Kiwamu A histochemical reporter system to study extracellular ATP response in plants |
title | A histochemical reporter system to study extracellular ATP response in plants |
title_full | A histochemical reporter system to study extracellular ATP response in plants |
title_fullStr | A histochemical reporter system to study extracellular ATP response in plants |
title_full_unstemmed | A histochemical reporter system to study extracellular ATP response in plants |
title_short | A histochemical reporter system to study extracellular ATP response in plants |
title_sort | histochemical reporter system to study extracellular atp response in plants |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272726/ https://www.ncbi.nlm.nih.gov/pubmed/37332691 http://dx.doi.org/10.3389/fpls.2023.1183335 |
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