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The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage

Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing Escherichia co...

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Autores principales: Snaith, Ann E., Dunn, Steven J., Moran, Robert A., Newton, Paul N., Dance, David A. B., Davong, Viengmon, Kuenzli, Esther, Kantele, Anu, Corander, Jukka, McNally, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272864/
https://www.ncbi.nlm.nih.gov/pubmed/37171860
http://dx.doi.org/10.1099/mgen.0.001000
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author Snaith, Ann E.
Dunn, Steven J.
Moran, Robert A.
Newton, Paul N.
Dance, David A. B.
Davong, Viengmon
Kuenzli, Esther
Kantele, Anu
Corander, Jukka
McNally, Alan
author_facet Snaith, Ann E.
Dunn, Steven J.
Moran, Robert A.
Newton, Paul N.
Dance, David A. B.
Davong, Viengmon
Kuenzli, Esther
Kantele, Anu
Corander, Jukka
McNally, Alan
author_sort Snaith, Ann E.
collection PubMed
description Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli that colonized travellers to Laos. Long-read sequencing was used to reconstruct complete plasmid sequences from 48 isolates obtained from the daily stool samples of 23 travellers over a 3 week period. This method revealed a collection of 105 distinct plasmids, 38.1 % (n=40) of which carried AMR genes. The plasmids in this population were diverse, mostly unreported and included 38 replicon types, with F-type plasmids (n=23) the most prevalent amongst those carrying AMR genes. Fine-scale analysis of all plasmids identified numerous AMR gene contexts and emphasized the importance of IS elements, specifically members of the IS6/IS26 family, in the evolution of complex multidrug resistance regions. We found a concerning convergence of ESBL and colistin resistance determinants, with three plasmids from two different F-type lineages carrying bla (CTX-M) and mcr genes. The extensive diversity seen here highlights the worrying probability that stable new vehicles for AMR will evolve in E. coli populations that can disseminate internationally through travel networks.
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spelling pubmed-102728642023-06-17 The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage Snaith, Ann E. Dunn, Steven J. Moran, Robert A. Newton, Paul N. Dance, David A. B. Davong, Viengmon Kuenzli, Esther Kantele, Anu Corander, Jukka McNally, Alan Microb Genom Research Articles Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli that colonized travellers to Laos. Long-read sequencing was used to reconstruct complete plasmid sequences from 48 isolates obtained from the daily stool samples of 23 travellers over a 3 week period. This method revealed a collection of 105 distinct plasmids, 38.1 % (n=40) of which carried AMR genes. The plasmids in this population were diverse, mostly unreported and included 38 replicon types, with F-type plasmids (n=23) the most prevalent amongst those carrying AMR genes. Fine-scale analysis of all plasmids identified numerous AMR gene contexts and emphasized the importance of IS elements, specifically members of the IS6/IS26 family, in the evolution of complex multidrug resistance regions. We found a concerning convergence of ESBL and colistin resistance determinants, with three plasmids from two different F-type lineages carrying bla (CTX-M) and mcr genes. The extensive diversity seen here highlights the worrying probability that stable new vehicles for AMR will evolve in E. coli populations that can disseminate internationally through travel networks. Microbiology Society 2023-05-12 /pmc/articles/PMC10272864/ /pubmed/37171860 http://dx.doi.org/10.1099/mgen.0.001000 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Research Articles
Snaith, Ann E.
Dunn, Steven J.
Moran, Robert A.
Newton, Paul N.
Dance, David A. B.
Davong, Viengmon
Kuenzli, Esther
Kantele, Anu
Corander, Jukka
McNally, Alan
The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title_full The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title_fullStr The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title_full_unstemmed The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title_short The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
title_sort highly diverse plasmid population found in escherichia coli colonizing travellers to laos and its role in antimicrobial resistance gene carriage
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272864/
https://www.ncbi.nlm.nih.gov/pubmed/37171860
http://dx.doi.org/10.1099/mgen.0.001000
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