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Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells

BACKGROUND: Amarogentin (AMA) is a secoiridoid glycoside extracted from Swertia and Gentiana roots and exhibits many biological effects such as antioxidative, anti‐inflammatory, and antitumor activities. Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by disorders in the regulat...

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Autores principales: Zhang, Qian, Wang, Hanlin, Ran, Cheng, Lyu, Yansi, Li, Fei, Yao, Yihang, Xing, Shaojun, Wang, Li, Chen, Si
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272903/
https://www.ncbi.nlm.nih.gov/pubmed/36131559
http://dx.doi.org/10.1002/ame2.12260
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author Zhang, Qian
Wang, Hanlin
Ran, Cheng
Lyu, Yansi
Li, Fei
Yao, Yihang
Xing, Shaojun
Wang, Li
Chen, Si
author_facet Zhang, Qian
Wang, Hanlin
Ran, Cheng
Lyu, Yansi
Li, Fei
Yao, Yihang
Xing, Shaojun
Wang, Li
Chen, Si
author_sort Zhang, Qian
collection PubMed
description BACKGROUND: Amarogentin (AMA) is a secoiridoid glycoside extracted from Swertia and Gentiana roots and exhibits many biological effects such as antioxidative, anti‐inflammatory, and antitumor activities. Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by disorders in the regulation of multiple inflammatory cytokines. No effective cure has been found for AD now. METHODS: We constructed the HaCat and splenocyte model and tested the inhibitory effect of AMA on IL‐4, IL‐6, and IL‐13 secretions using enzyme‐linked immunosorbent assay (ELISA). The AD mouse model was constructed and treated with AMA, the severity of skin lesions was observed, epidermal tissue was collected, and epidermal thickness and mast cell infiltration were observed using hematoxylin and eosin and toluidine blue staining, respectively. The expression of kallikrein‐related peptidase 7 (KLK7) and filaggrin (FLG) was detected using immunostaining and Western blot analysis. The mRNA expression of KLK7 and FLG was detected using quantitative polymerase chain reaction (qPCR). Blood immunoglobulin E (IgE) secretion was detected. RESULTS: AMA inhibited IL‐6 secreted by tumor necrosis factor (TNF)‐α‐induced HaCaT cells and reduced IL‐4 and IL‐13 secreted by phytohemagglutinin (PHA)‐induced primary cells in the mice spleen. It was found that the treatment of AMA with 2,4‐dinitrochlorobenzene‐induced AD‐like mice could promote the recovery of dermatitis, reduce the score of dermatitis severity and the scratching frequency, treat the skin lesions, reduce the epidermal thickness, decrease the infiltration of mast cells, reduce the IgE level in serum, decrease the expression levels of AD‐related cytokines, increase protein and mRNA expression of FLG, and reduce the protein and mRNA expression of KLK7 in the skin tissues of AD‐like mice. CONCLUSION: In conclusion, AMA inhibits inflammatory response at the cellular level, and AMA reduces the validation response of specific dermatitis mice, relieves pruritus, and repairs the damaged skin barrier.
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spelling pubmed-102729032023-06-17 Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells Zhang, Qian Wang, Hanlin Ran, Cheng Lyu, Yansi Li, Fei Yao, Yihang Xing, Shaojun Wang, Li Chen, Si Animal Model Exp Med Regular Articles BACKGROUND: Amarogentin (AMA) is a secoiridoid glycoside extracted from Swertia and Gentiana roots and exhibits many biological effects such as antioxidative, anti‐inflammatory, and antitumor activities. Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by disorders in the regulation of multiple inflammatory cytokines. No effective cure has been found for AD now. METHODS: We constructed the HaCat and splenocyte model and tested the inhibitory effect of AMA on IL‐4, IL‐6, and IL‐13 secretions using enzyme‐linked immunosorbent assay (ELISA). The AD mouse model was constructed and treated with AMA, the severity of skin lesions was observed, epidermal tissue was collected, and epidermal thickness and mast cell infiltration were observed using hematoxylin and eosin and toluidine blue staining, respectively. The expression of kallikrein‐related peptidase 7 (KLK7) and filaggrin (FLG) was detected using immunostaining and Western blot analysis. The mRNA expression of KLK7 and FLG was detected using quantitative polymerase chain reaction (qPCR). Blood immunoglobulin E (IgE) secretion was detected. RESULTS: AMA inhibited IL‐6 secreted by tumor necrosis factor (TNF)‐α‐induced HaCaT cells and reduced IL‐4 and IL‐13 secreted by phytohemagglutinin (PHA)‐induced primary cells in the mice spleen. It was found that the treatment of AMA with 2,4‐dinitrochlorobenzene‐induced AD‐like mice could promote the recovery of dermatitis, reduce the score of dermatitis severity and the scratching frequency, treat the skin lesions, reduce the epidermal thickness, decrease the infiltration of mast cells, reduce the IgE level in serum, decrease the expression levels of AD‐related cytokines, increase protein and mRNA expression of FLG, and reduce the protein and mRNA expression of KLK7 in the skin tissues of AD‐like mice. CONCLUSION: In conclusion, AMA inhibits inflammatory response at the cellular level, and AMA reduces the validation response of specific dermatitis mice, relieves pruritus, and repairs the damaged skin barrier. John Wiley and Sons Inc. 2022-09-21 /pmc/articles/PMC10272903/ /pubmed/36131559 http://dx.doi.org/10.1002/ame2.12260 Text en © 2022 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Regular Articles
Zhang, Qian
Wang, Hanlin
Ran, Cheng
Lyu, Yansi
Li, Fei
Yao, Yihang
Xing, Shaojun
Wang, Li
Chen, Si
Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title_full Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title_fullStr Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title_full_unstemmed Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title_short Anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in HaCat cells
title_sort anti‐inflammatory effects of amarogentin on 2,4‐dinitrochlorobenzene‐induced atopic dermatitis–like mice and in hacat cells
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10272903/
https://www.ncbi.nlm.nih.gov/pubmed/36131559
http://dx.doi.org/10.1002/ame2.12260
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