A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells

[Image: see text] Bioorthogonal deprotections are readily used to control biological function in a cell-specific manner. To further improve the spatial resolution of these reactions, we here present a lysosome-targeted tetrazine for an organelle-specific deprotection reaction. We show that trans-cyc...

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Autores principales: Ligthart, Nina A.M., de Geus, Mark A.R., van de Plassche, Merel A.T., Torres García, Diana, Isendoorn, Marjolein M.E., Reinalda, Luuk, Ofman, Daniëlle, van Leeuwen, Tyrza, van Kasteren, Sander I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10273227/
https://www.ncbi.nlm.nih.gov/pubmed/37269296
http://dx.doi.org/10.1021/jacs.3c02139
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author Ligthart, Nina A.M.
de Geus, Mark A.R.
van de Plassche, Merel A.T.
Torres García, Diana
Isendoorn, Marjolein M.E.
Reinalda, Luuk
Ofman, Daniëlle
van Leeuwen, Tyrza
van Kasteren, Sander I.
author_facet Ligthart, Nina A.M.
de Geus, Mark A.R.
van de Plassche, Merel A.T.
Torres García, Diana
Isendoorn, Marjolein M.E.
Reinalda, Luuk
Ofman, Daniëlle
van Leeuwen, Tyrza
van Kasteren, Sander I.
author_sort Ligthart, Nina A.M.
collection PubMed
description [Image: see text] Bioorthogonal deprotections are readily used to control biological function in a cell-specific manner. To further improve the spatial resolution of these reactions, we here present a lysosome-targeted tetrazine for an organelle-specific deprotection reaction. We show that trans-cyclooctene deprotection with this reagent can be used to control the biological activity of ligands for invariant natural killer T cells in the lysosome to shed light on the processing pathway in antigen presenting cells. We then use the lysosome-targeted tetrazine to show that long peptide antigens used for CD8(+) T cell activation do not pass through this organelle, suggesting a role for the earlier endosomal compartments for their processing.
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spelling pubmed-102732272023-06-17 A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells Ligthart, Nina A.M. de Geus, Mark A.R. van de Plassche, Merel A.T. Torres García, Diana Isendoorn, Marjolein M.E. Reinalda, Luuk Ofman, Daniëlle van Leeuwen, Tyrza van Kasteren, Sander I. J Am Chem Soc [Image: see text] Bioorthogonal deprotections are readily used to control biological function in a cell-specific manner. To further improve the spatial resolution of these reactions, we here present a lysosome-targeted tetrazine for an organelle-specific deprotection reaction. We show that trans-cyclooctene deprotection with this reagent can be used to control the biological activity of ligands for invariant natural killer T cells in the lysosome to shed light on the processing pathway in antigen presenting cells. We then use the lysosome-targeted tetrazine to show that long peptide antigens used for CD8(+) T cell activation do not pass through this organelle, suggesting a role for the earlier endosomal compartments for their processing. American Chemical Society 2023-06-03 /pmc/articles/PMC10273227/ /pubmed/37269296 http://dx.doi.org/10.1021/jacs.3c02139 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Ligthart, Nina A.M.
de Geus, Mark A.R.
van de Plassche, Merel A.T.
Torres García, Diana
Isendoorn, Marjolein M.E.
Reinalda, Luuk
Ofman, Daniëlle
van Leeuwen, Tyrza
van Kasteren, Sander I.
A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title_full A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title_fullStr A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title_full_unstemmed A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title_short A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells
title_sort lysosome-targeted tetrazine for organelle-specific click-to-release chemistry in antigen presenting cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10273227/
https://www.ncbi.nlm.nih.gov/pubmed/37269296
http://dx.doi.org/10.1021/jacs.3c02139
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