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Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia
Environmental risk assessment is a critical tool for protecting biodiversity and its effectiveness is predicated on predicting how natural populations respond to environmental stressors. Yet, routine toxicity testing typically examines only one genotype, which may render risk assessments inaccurate...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274664/ https://www.ncbi.nlm.nih.gov/pubmed/37333160 http://dx.doi.org/10.1101/2023.06.06.543817 |
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author | Shahmohamadloo, René S. Rudman, Seth M. Clare, Catherine I. Westrick, Judy A. De Meester, Luc Fryxell, John M. |
author_facet | Shahmohamadloo, René S. Rudman, Seth M. Clare, Catherine I. Westrick, Judy A. De Meester, Luc Fryxell, John M. |
author_sort | Shahmohamadloo, René S. |
collection | PubMed |
description | Environmental risk assessment is a critical tool for protecting biodiversity and its effectiveness is predicated on predicting how natural populations respond to environmental stressors. Yet, routine toxicity testing typically examines only one genotype, which may render risk assessments inaccurate at the population scale. To determine the importance of intraspecific variation in the translation of toxicity testing to populations, we quantified the magnitude of genetic variation within 20 Daphnia magna clones derived from one lake using whole genome sequencing and phenotypic assays. We repeated these assays across two exposure levels of Microcystis aeruginosa, a cosmopolitan freshwater contaminant. We found considerable intraspecific genetic variation in survival, growth, and reproduction. Exposure to Microcystis amplified the degree of intraspecific variation. Simulations demonstrate assays employing one clone failed to produce an estimate within the 95% confidence interval over half of the time. These results illuminate the importance of incorporating intraspecific genetic variation, but not necessarily genome sequences, into toxicity testing to reliably predict how natural populations will respond to environmental stressors. |
format | Online Article Text |
id | pubmed-10274664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-102746642023-06-17 Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia Shahmohamadloo, René S. Rudman, Seth M. Clare, Catherine I. Westrick, Judy A. De Meester, Luc Fryxell, John M. bioRxiv Article Environmental risk assessment is a critical tool for protecting biodiversity and its effectiveness is predicated on predicting how natural populations respond to environmental stressors. Yet, routine toxicity testing typically examines only one genotype, which may render risk assessments inaccurate at the population scale. To determine the importance of intraspecific variation in the translation of toxicity testing to populations, we quantified the magnitude of genetic variation within 20 Daphnia magna clones derived from one lake using whole genome sequencing and phenotypic assays. We repeated these assays across two exposure levels of Microcystis aeruginosa, a cosmopolitan freshwater contaminant. We found considerable intraspecific genetic variation in survival, growth, and reproduction. Exposure to Microcystis amplified the degree of intraspecific variation. Simulations demonstrate assays employing one clone failed to produce an estimate within the 95% confidence interval over half of the time. These results illuminate the importance of incorporating intraspecific genetic variation, but not necessarily genome sequences, into toxicity testing to reliably predict how natural populations will respond to environmental stressors. Cold Spring Harbor Laboratory 2023-06-07 /pmc/articles/PMC10274664/ /pubmed/37333160 http://dx.doi.org/10.1101/2023.06.06.543817 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Shahmohamadloo, René S. Rudman, Seth M. Clare, Catherine I. Westrick, Judy A. De Meester, Luc Fryxell, John M. Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title | Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title_full | Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title_fullStr | Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title_full_unstemmed | Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title_short | Intraspecific genetic variation is critical to robust toxicological predictions in Daphnia |
title_sort | intraspecific genetic variation is critical to robust toxicological predictions in daphnia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274664/ https://www.ncbi.nlm.nih.gov/pubmed/37333160 http://dx.doi.org/10.1101/2023.06.06.543817 |
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