A SMART method for efficiently isolating monoclonal antibodies from individual rhesus macaque memory B cells

Characterizing antigen-specific B cells is a critical component of vaccine and infectious disease studies in rhesus macaques (RMs). However, it is challenging to capture immunoglobulin variable (IgV) genes from individual RM B cells using 5’ multiplex (MTPX) primers in nested PCR reactions. In parti...

Descripción completa

Detalles Bibliográficos
Autores principales: Weinfurter, Jason T., Bennett, Sarah N., Reynolds, Matthew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274751/
https://www.ncbi.nlm.nih.gov/pubmed/37333083
http://dx.doi.org/10.1101/2023.06.02.543510

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274751/
https://www.ncbi.nlm.nih.gov/pubmed/37333083
http://dx.doi.org/10.1101/2023.06.02.543510

Ejemplares similares