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A SMART method for efficiently isolating monoclonal antibodies from individual rhesus macaque memory B cells

Characterizing antigen-specific B cells is a critical component of vaccine and infectious disease studies in rhesus macaques (RMs). However, it is challenging to capture immunoglobulin variable (IgV) genes from individual RM B cells using 5’ multiplex (MTPX) primers in nested PCR reactions. In parti...

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Detalles Bibliográficos
Autores principales:	Weinfurter, Jason T., Bennett, Sarah N., Reynolds, Matthew
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10274751/ https://www.ncbi.nlm.nih.gov/pubmed/37333083 http://dx.doi.org/10.1101/2023.06.02.543510

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