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Anionic G•U pairs in bacterial ribosomal rRNAs

Wobble GU pairs (or G•U) occur frequently within double-stranded RNA helices interspersed between standard G=C and A-U Watson–Crick pairs. Another type of G•U pair interacting via their Watson–Crick edges has been observed in the A site of ribosome structures between a modified U34 in the tRNA antic...

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Autores principales: Westhof, Eric, Watson, Zoe L., Zirbel, Craig L., Cate, Jamie H.D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275268/
https://www.ncbi.nlm.nih.gov/pubmed/37068913
http://dx.doi.org/10.1261/rna.079583.123
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author Westhof, Eric
Watson, Zoe L.
Zirbel, Craig L.
Cate, Jamie H.D.
author_facet Westhof, Eric
Watson, Zoe L.
Zirbel, Craig L.
Cate, Jamie H.D.
author_sort Westhof, Eric
collection PubMed
description Wobble GU pairs (or G•U) occur frequently within double-stranded RNA helices interspersed between standard G=C and A-U Watson–Crick pairs. Another type of G•U pair interacting via their Watson–Crick edges has been observed in the A site of ribosome structures between a modified U34 in the tRNA anticodon triplet and G + 3 in the mRNA. In such pairs, the electronic structure of the U is changed with a negative charge on N3(U), resulting in two H-bonds between N1(G)…O4(U) and N2(G)…N3(U). Here, we report that such pairs occur in other highly conserved positions in ribosomal RNAs of bacteria in the absence of U modification. An anionic cis Watson–Crick G•G pair is also observed and well conserved in the small subunit. These pairs are observed in tightly folded regions.
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spelling pubmed-102752682023-07-01 Anionic G•U pairs in bacterial ribosomal rRNAs Westhof, Eric Watson, Zoe L. Zirbel, Craig L. Cate, Jamie H.D. RNA Articles Wobble GU pairs (or G•U) occur frequently within double-stranded RNA helices interspersed between standard G=C and A-U Watson–Crick pairs. Another type of G•U pair interacting via their Watson–Crick edges has been observed in the A site of ribosome structures between a modified U34 in the tRNA anticodon triplet and G + 3 in the mRNA. In such pairs, the electronic structure of the U is changed with a negative charge on N3(U), resulting in two H-bonds between N1(G)…O4(U) and N2(G)…N3(U). Here, we report that such pairs occur in other highly conserved positions in ribosomal RNAs of bacteria in the absence of U modification. An anionic cis Watson–Crick G•G pair is also observed and well conserved in the small subunit. These pairs are observed in tightly folded regions. Cold Spring Harbor Laboratory Press 2023-07 /pmc/articles/PMC10275268/ /pubmed/37068913 http://dx.doi.org/10.1261/rna.079583.123 Text en © 2023 Westhof et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Articles
Westhof, Eric
Watson, Zoe L.
Zirbel, Craig L.
Cate, Jamie H.D.
Anionic G•U pairs in bacterial ribosomal rRNAs
title Anionic G•U pairs in bacterial ribosomal rRNAs
title_full Anionic G•U pairs in bacterial ribosomal rRNAs
title_fullStr Anionic G•U pairs in bacterial ribosomal rRNAs
title_full_unstemmed Anionic G•U pairs in bacterial ribosomal rRNAs
title_short Anionic G•U pairs in bacterial ribosomal rRNAs
title_sort anionic g•u pairs in bacterial ribosomal rrnas
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275268/
https://www.ncbi.nlm.nih.gov/pubmed/37068913
http://dx.doi.org/10.1261/rna.079583.123
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