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Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response
Biomedical devices comprise a major component of modern medicine, however immune-mediated fibrosis and rejection can limit their function over time. Here, we describe a humanized mouse model that recapitulates fibrosis following biomaterial implantation. Cellular and cytokine responses to multiple b...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275594/ https://www.ncbi.nlm.nih.gov/pubmed/37327334 http://dx.doi.org/10.1126/sciadv.ade9488 |
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author | Doloff, Joshua C. Ma, Minglin Sadraei, Atieh Tam, Hok Hei Farah, Shady Hollister-Lock, Jennifer Vegas, Arturo J. Veiseh, Omid Quiroz, Victor M. Rakoski, Amanda Aresta-DaSilva, Stephanie Bader, Andrew R. Griffin, Marissa Weir, Gordon C. Brehm, Michael A. Shultz, Leonard D. Langer, Robert Greiner, Dale L. Anderson, Daniel G. |
author_facet | Doloff, Joshua C. Ma, Minglin Sadraei, Atieh Tam, Hok Hei Farah, Shady Hollister-Lock, Jennifer Vegas, Arturo J. Veiseh, Omid Quiroz, Victor M. Rakoski, Amanda Aresta-DaSilva, Stephanie Bader, Andrew R. Griffin, Marissa Weir, Gordon C. Brehm, Michael A. Shultz, Leonard D. Langer, Robert Greiner, Dale L. Anderson, Daniel G. |
author_sort | Doloff, Joshua C. |
collection | PubMed |
description | Biomedical devices comprise a major component of modern medicine, however immune-mediated fibrosis and rejection can limit their function over time. Here, we describe a humanized mouse model that recapitulates fibrosis following biomaterial implantation. Cellular and cytokine responses to multiple biomaterials were evaluated across different implant sites. Human innate immune macrophages were verified as essential to biomaterial rejection in this model and were capable of cross-talk with mouse fibroblasts for collagen matrix deposition. Cytokine and cytokine receptor array analysis confirmed core signaling in the fibrotic cascade. Foreign body giant cell formation, often unobserved in mice, was also prominent. Last, high-resolution microscopy coupled with multiplexed antibody capture digital profiling analysis supplied spatial resolution of rejection responses. This model enables the study of human immune cell–mediated fibrosis and interactions with implanted biomaterials and devices. |
format | Online Article Text |
id | pubmed-10275594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102755942023-06-17 Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response Doloff, Joshua C. Ma, Minglin Sadraei, Atieh Tam, Hok Hei Farah, Shady Hollister-Lock, Jennifer Vegas, Arturo J. Veiseh, Omid Quiroz, Victor M. Rakoski, Amanda Aresta-DaSilva, Stephanie Bader, Andrew R. Griffin, Marissa Weir, Gordon C. Brehm, Michael A. Shultz, Leonard D. Langer, Robert Greiner, Dale L. Anderson, Daniel G. Sci Adv Biomedicine and Life Sciences Biomedical devices comprise a major component of modern medicine, however immune-mediated fibrosis and rejection can limit their function over time. Here, we describe a humanized mouse model that recapitulates fibrosis following biomaterial implantation. Cellular and cytokine responses to multiple biomaterials were evaluated across different implant sites. Human innate immune macrophages were verified as essential to biomaterial rejection in this model and were capable of cross-talk with mouse fibroblasts for collagen matrix deposition. Cytokine and cytokine receptor array analysis confirmed core signaling in the fibrotic cascade. Foreign body giant cell formation, often unobserved in mice, was also prominent. Last, high-resolution microscopy coupled with multiplexed antibody capture digital profiling analysis supplied spatial resolution of rejection responses. This model enables the study of human immune cell–mediated fibrosis and interactions with implanted biomaterials and devices. American Association for the Advancement of Science 2023-06-16 /pmc/articles/PMC10275594/ /pubmed/37327334 http://dx.doi.org/10.1126/sciadv.ade9488 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Doloff, Joshua C. Ma, Minglin Sadraei, Atieh Tam, Hok Hei Farah, Shady Hollister-Lock, Jennifer Vegas, Arturo J. Veiseh, Omid Quiroz, Victor M. Rakoski, Amanda Aresta-DaSilva, Stephanie Bader, Andrew R. Griffin, Marissa Weir, Gordon C. Brehm, Michael A. Shultz, Leonard D. Langer, Robert Greiner, Dale L. Anderson, Daniel G. Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title | Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title_full | Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title_fullStr | Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title_full_unstemmed | Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title_short | Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
title_sort | identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275594/ https://www.ncbi.nlm.nih.gov/pubmed/37327334 http://dx.doi.org/10.1126/sciadv.ade9488 |
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