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Ribozyme activity modulates the physical properties of RNA–peptide coacervates
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology. In each of these fields, the development of model systems with varied and tuneable material properties is of great...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275638/ https://www.ncbi.nlm.nih.gov/pubmed/37326308 http://dx.doi.org/10.7554/eLife.83543 |
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author | Le Vay, Kristian Kyle Salibi, Elia Ghosh, Basusree Tang, TY Dora Mutschler, Hannes |
author_facet | Le Vay, Kristian Kyle Salibi, Elia Ghosh, Basusree Tang, TY Dora Mutschler, Hannes |
author_sort | Le Vay, Kristian Kyle |
collection | PubMed |
description | Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology. In each of these fields, the development of model systems with varied and tuneable material properties is of great importance for replicating properties of life. Here, we develop a ligase ribozyme system capable of concatenating short RNA fragments into long chains. Our results show that the formation of coacervate microdroplets with the ligase ribozyme and poly(L-lysine) enhances ribozyme rate and yield, which in turn increases the length of the anionic polymer component of the system and imparts specific physical properties to the droplets. Droplets containing active ribozyme sequences resist growth, do not wet or spread on unpassivated surfaces, and exhibit reduced transfer of RNA between droplets when compared to controls containing inactive sequences. These altered behaviours, which stem from RNA sequence and catalytic activity, constitute a specific phenotype and potential fitness advantage, opening the door to selection and evolution experiments based on a genotype–phenotype linkage. |
format | Online Article Text |
id | pubmed-10275638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-102756382023-06-17 Ribozyme activity modulates the physical properties of RNA–peptide coacervates Le Vay, Kristian Kyle Salibi, Elia Ghosh, Basusree Tang, TY Dora Mutschler, Hannes eLife Biochemistry and Chemical Biology Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology. In each of these fields, the development of model systems with varied and tuneable material properties is of great importance for replicating properties of life. Here, we develop a ligase ribozyme system capable of concatenating short RNA fragments into long chains. Our results show that the formation of coacervate microdroplets with the ligase ribozyme and poly(L-lysine) enhances ribozyme rate and yield, which in turn increases the length of the anionic polymer component of the system and imparts specific physical properties to the droplets. Droplets containing active ribozyme sequences resist growth, do not wet or spread on unpassivated surfaces, and exhibit reduced transfer of RNA between droplets when compared to controls containing inactive sequences. These altered behaviours, which stem from RNA sequence and catalytic activity, constitute a specific phenotype and potential fitness advantage, opening the door to selection and evolution experiments based on a genotype–phenotype linkage. eLife Sciences Publications, Ltd 2023-06-16 /pmc/articles/PMC10275638/ /pubmed/37326308 http://dx.doi.org/10.7554/eLife.83543 Text en © 2023, Le Vay, Salibi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Le Vay, Kristian Kyle Salibi, Elia Ghosh, Basusree Tang, TY Dora Mutschler, Hannes Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title | Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title_full | Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title_fullStr | Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title_full_unstemmed | Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title_short | Ribozyme activity modulates the physical properties of RNA–peptide coacervates |
title_sort | ribozyme activity modulates the physical properties of rna–peptide coacervates |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10275638/ https://www.ncbi.nlm.nih.gov/pubmed/37326308 http://dx.doi.org/10.7554/eLife.83543 |
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