Hybrid-DIA: intelligent data acquisition integrates targeted and discovery proteomics to analyze phospho-signaling in single spheroids

Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-based phosphoproteomics for signaling pathway reconstitution is challenging, especially when analyzing tiny sample amounts. To address this, we present a hybrid data-independent acquisition (DIA) strategy (hy...

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Detalles Bibliográficos
Autores principales: Martínez-Val, Ana, Fort, Kyle, Koenig, Claire, Van der Hoeven, Leander, Franciosa, Giulia, Moehring, Thomas, Ishihama, Yasushi, Chen, Yu-ju, Makarov, Alexander, Xuan, Yue, Olsen, Jesper V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276052/
https://www.ncbi.nlm.nih.gov/pubmed/37328457
http://dx.doi.org/10.1038/s41467-023-39347-y

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