Evaluating the function of murine quiescent hematopoietic stem cells following non-homologous end joining-based genome editing

Preculture is indispensable for achieving highly efficient non-homologous end joining (NHEJ)-based genome editing. Here, we present a protocol for optimizing genome editing conditions for murine hematopoietic stem cells (HSCs) and evaluating their function following NHEJ-based genome editing. We des...

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Detalles Bibliográficos
Autores principales: Shiroshita, Kohei, Kobayashi, Hiroshi, Takubo, Keiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276276/
https://www.ncbi.nlm.nih.gov/pubmed/37300828
http://dx.doi.org/10.1016/j.xpro.2023.102347

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276276/
https://www.ncbi.nlm.nih.gov/pubmed/37300828
http://dx.doi.org/10.1016/j.xpro.2023.102347

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