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Evaluating the function of murine quiescent hematopoietic stem cells following non-homologous end joining-based genome editing
Preculture is indispensable for achieving highly efficient non-homologous end joining (NHEJ)-based genome editing. Here, we present a protocol for optimizing genome editing conditions for murine hematopoietic stem cells (HSCs) and evaluating their function following NHEJ-based genome editing. We des...
Autores principales: | Shiroshita, Kohei, Kobayashi, Hiroshi, Takubo, Keiyo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276276/ https://www.ncbi.nlm.nih.gov/pubmed/37300828 http://dx.doi.org/10.1016/j.xpro.2023.102347 |
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