Protocol for the generation and automated confocal imaging of whole multi-cellular tumor spheroids

Multi-cellular tumor spheroids (MCTS) have found widespread use in pre-clinical research. However, their complex three-dimensional structure makes immunofluorescent staining and imaging challenging. Here, we present a protocol for whole spheroid staining and automated imaging using laser-scanning co...

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Detalles Bibliográficos
Autores principales: Genenger, Benjamin, McAlary, Luke, Perry, Jay R., Ashford, Bruce, Ranson, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276290/
https://www.ncbi.nlm.nih.gov/pubmed/37300829
http://dx.doi.org/10.1016/j.xpro.2023.102331

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276290/
https://www.ncbi.nlm.nih.gov/pubmed/37300829
http://dx.doi.org/10.1016/j.xpro.2023.102331

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