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Protocol for the generation and automated confocal imaging of whole multi-cellular tumor spheroids
Multi-cellular tumor spheroids (MCTS) have found widespread use in pre-clinical research. However, their complex three-dimensional structure makes immunofluorescent staining and imaging challenging. Here, we present a protocol for whole spheroid staining and automated imaging using laser-scanning co...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276290/ https://www.ncbi.nlm.nih.gov/pubmed/37300829 http://dx.doi.org/10.1016/j.xpro.2023.102331 |