Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen

This work reported an electrochemical method for the detection of SARS-CoV-2 major protease (Mpro). Specifically, ferrocene (Fc)-labeled peptide substrates were immobilized on the gold nanoparticles (AuNPs)-modified electrode. Cleavage of the peptides by Mpro led to the release of Fc tags and the de...

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Detalles Bibliográficos
Autores principales: Zhang, Qiongyu, Liu, Gang, Ou, Lingbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by ESG. Published by Elsevier B.V. 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276345/
https://www.ncbi.nlm.nih.gov/pubmed/37359208
http://dx.doi.org/10.20964/2022.04.19
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author Zhang, Qiongyu
Liu, Gang
Ou, Lingbin
author_facet Zhang, Qiongyu
Liu, Gang
Ou, Lingbin
author_sort Zhang, Qiongyu
collection PubMed
description This work reported an electrochemical method for the detection of SARS-CoV-2 major protease (Mpro). Specifically, ferrocene (Fc)-labeled peptide substrates were immobilized on the gold nanoparticles (AuNPs)-modified electrode. Cleavage of the peptides by Mpro led to the release of Fc tags and the decrease of the electrochemical signals. The analytical performance of the biosensor for analysis of Mpro was investigated. Inhibiting the activity of Mpro prevented the cleavage of the peptide substrates. The method was successfully used to evaluate the inhibition efficiency of a well-known inhibitor.
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spelling pubmed-102763452023-06-21 Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen Zhang, Qiongyu Liu, Gang Ou, Lingbin Int J Electrochem Sci Short Communication This work reported an electrochemical method for the detection of SARS-CoV-2 major protease (Mpro). Specifically, ferrocene (Fc)-labeled peptide substrates were immobilized on the gold nanoparticles (AuNPs)-modified electrode. Cleavage of the peptides by Mpro led to the release of Fc tags and the decrease of the electrochemical signals. The analytical performance of the biosensor for analysis of Mpro was investigated. Inhibiting the activity of Mpro prevented the cleavage of the peptide substrates. The method was successfully used to evaluate the inhibition efficiency of a well-known inhibitor. The Authors. Published by ESG. Published by Elsevier B.V. 2022-04 2023-06-17 /pmc/articles/PMC10276345/ /pubmed/37359208 http://dx.doi.org/10.20964/2022.04.19 Text en © 2022 The Authors. Published by ESG Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Zhang, Qiongyu
Liu, Gang
Ou, Lingbin
Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title_full Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title_fullStr Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title_full_unstemmed Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title_short Electrochemical Biosensor for the Detection of SARS-CoV-2 Main Protease and Its Inhibitor Ebselen
title_sort electrochemical biosensor for the detection of sars-cov-2 main protease and its inhibitor ebselen
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276345/
https://www.ncbi.nlm.nih.gov/pubmed/37359208
http://dx.doi.org/10.20964/2022.04.19
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AT liugang electrochemicalbiosensorforthedetectionofsarscov2mainproteaseanditsinhibitorebselen
AT oulingbin electrochemicalbiosensorforthedetectionofsarscov2mainproteaseanditsinhibitorebselen

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