Cargando…

Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows

We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-l...

Descripción completa

Detalles Bibliográficos
Autores principales: Almeida, Kleves V, Resende, Tales L, Silva, Luiz Henrique P, Dorich, Christopher D, Pereira, Andre B D, Soder, Kathy J, Brito, Andre F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276549/
https://www.ncbi.nlm.nih.gov/pubmed/37334244
http://dx.doi.org/10.1093/tas/txad050
_version_ 1785060102649151488
author Almeida, Kleves V
Resende, Tales L
Silva, Luiz Henrique P
Dorich, Christopher D
Pereira, Andre B D
Soder, Kathy J
Brito, Andre F
author_facet Almeida, Kleves V
Resende, Tales L
Silva, Luiz Henrique P
Dorich, Christopher D
Pereira, Andre B D
Soder, Kathy J
Brito, Andre F
author_sort Almeida, Kleves V
collection PubMed
description We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-lactation Jersey cows were used in the study. Of these 20 cows, 12 were used for ruminal sampling, 16 for enteric CH(4) measurements, and all for spot urine collection. Each period lasted 21 d with 14 d for diet adaptation and 7 d for data and sample collection. Diets were formulated by replacing corn meal and soybean meal with 0%, 5%, 10%, and 15% of GFX in the diet’s dry matter. Ruminal fluid samples obtained via stomach tubing were used for DNA extraction. Enteric CH(4) production was measured using the sulfur hexafluoride tracer technique. Diets had no effect on ruminal microbiota diversity. Similarly, the relative abundance of ruminal archaea genera was not affected by diets. In contrast, GFX decreased or increased linearly the relative abundance of Firmicutes (P < 0.01) and Bacteroidetes (P < 0.01), respectively. The relative abundance of the ruminal bacteria Ruminococcus (P < 0.01) and Clostridium (P < 0.01) decreased linearly, and that of Prevotella (P < 0.01) and Pseudobutyrivibrio (P < 0.01) increased linearly with feeding GFX. A tendency for a linear reduction (P = 0.055) in enteric CH(4) production (from 304 to 256 g/d) was observed in cows fed increasing amounts of GFX. However, neither CH(4) yield nor CH(4) intensity was affected by treatments. Diets had no effect on the urinary excretion of uric acid, allantoin, and total PD. Overall, feeding GFX decreased linearly the relative abundance of the ruminal bacterial genera Ruminococcus and Clostridium and enteric CH(4) production, but no change was seen for CH(4) yield and CH(4) intensity, or urinary excretion of total PD, suggesting no detrimental effect of GFX on microbial protein synthesis in the rumen.
format Online
Article
Text
id pubmed-10276549
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-102765492023-06-18 Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows Almeida, Kleves V Resende, Tales L Silva, Luiz Henrique P Dorich, Christopher D Pereira, Andre B D Soder, Kathy J Brito, Andre F Transl Anim Sci Ruminant Nutrition We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-lactation Jersey cows were used in the study. Of these 20 cows, 12 were used for ruminal sampling, 16 for enteric CH(4) measurements, and all for spot urine collection. Each period lasted 21 d with 14 d for diet adaptation and 7 d for data and sample collection. Diets were formulated by replacing corn meal and soybean meal with 0%, 5%, 10%, and 15% of GFX in the diet’s dry matter. Ruminal fluid samples obtained via stomach tubing were used for DNA extraction. Enteric CH(4) production was measured using the sulfur hexafluoride tracer technique. Diets had no effect on ruminal microbiota diversity. Similarly, the relative abundance of ruminal archaea genera was not affected by diets. In contrast, GFX decreased or increased linearly the relative abundance of Firmicutes (P < 0.01) and Bacteroidetes (P < 0.01), respectively. The relative abundance of the ruminal bacteria Ruminococcus (P < 0.01) and Clostridium (P < 0.01) decreased linearly, and that of Prevotella (P < 0.01) and Pseudobutyrivibrio (P < 0.01) increased linearly with feeding GFX. A tendency for a linear reduction (P = 0.055) in enteric CH(4) production (from 304 to 256 g/d) was observed in cows fed increasing amounts of GFX. However, neither CH(4) yield nor CH(4) intensity was affected by treatments. Diets had no effect on the urinary excretion of uric acid, allantoin, and total PD. Overall, feeding GFX decreased linearly the relative abundance of the ruminal bacterial genera Ruminococcus and Clostridium and enteric CH(4) production, but no change was seen for CH(4) yield and CH(4) intensity, or urinary excretion of total PD, suggesting no detrimental effect of GFX on microbial protein synthesis in the rumen. Oxford University Press 2023-05-29 /pmc/articles/PMC10276549/ /pubmed/37334244 http://dx.doi.org/10.1093/tas/txad050 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Ruminant Nutrition
Almeida, Kleves V
Resende, Tales L
Silva, Luiz Henrique P
Dorich, Christopher D
Pereira, Andre B D
Soder, Kathy J
Brito, Andre F
Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title_full Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title_fullStr Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title_full_unstemmed Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title_short Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
title_sort feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
topic Ruminant Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276549/
https://www.ncbi.nlm.nih.gov/pubmed/37334244
http://dx.doi.org/10.1093/tas/txad050
work_keys_str_mv AT almeidaklevesv feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT resendetalesl feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT silvaluizhenriquep feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT dorichchristopherd feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT pereiraandrebd feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT soderkathyj feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows
AT britoandref feedingincrementalamountsofgroundflaxseedeffectsondiversityandrelativeabundanceofruminalmicrobiotaandentericmethaneemissionsinlactatingdairycows