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Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows
We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-l...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276549/ https://www.ncbi.nlm.nih.gov/pubmed/37334244 http://dx.doi.org/10.1093/tas/txad050 |
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author | Almeida, Kleves V Resende, Tales L Silva, Luiz Henrique P Dorich, Christopher D Pereira, Andre B D Soder, Kathy J Brito, Andre F |
author_facet | Almeida, Kleves V Resende, Tales L Silva, Luiz Henrique P Dorich, Christopher D Pereira, Andre B D Soder, Kathy J Brito, Andre F |
author_sort | Almeida, Kleves V |
collection | PubMed |
description | We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-lactation Jersey cows were used in the study. Of these 20 cows, 12 were used for ruminal sampling, 16 for enteric CH(4) measurements, and all for spot urine collection. Each period lasted 21 d with 14 d for diet adaptation and 7 d for data and sample collection. Diets were formulated by replacing corn meal and soybean meal with 0%, 5%, 10%, and 15% of GFX in the diet’s dry matter. Ruminal fluid samples obtained via stomach tubing were used for DNA extraction. Enteric CH(4) production was measured using the sulfur hexafluoride tracer technique. Diets had no effect on ruminal microbiota diversity. Similarly, the relative abundance of ruminal archaea genera was not affected by diets. In contrast, GFX decreased or increased linearly the relative abundance of Firmicutes (P < 0.01) and Bacteroidetes (P < 0.01), respectively. The relative abundance of the ruminal bacteria Ruminococcus (P < 0.01) and Clostridium (P < 0.01) decreased linearly, and that of Prevotella (P < 0.01) and Pseudobutyrivibrio (P < 0.01) increased linearly with feeding GFX. A tendency for a linear reduction (P = 0.055) in enteric CH(4) production (from 304 to 256 g/d) was observed in cows fed increasing amounts of GFX. However, neither CH(4) yield nor CH(4) intensity was affected by treatments. Diets had no effect on the urinary excretion of uric acid, allantoin, and total PD. Overall, feeding GFX decreased linearly the relative abundance of the ruminal bacterial genera Ruminococcus and Clostridium and enteric CH(4) production, but no change was seen for CH(4) yield and CH(4) intensity, or urinary excretion of total PD, suggesting no detrimental effect of GFX on microbial protein synthesis in the rumen. |
format | Online Article Text |
id | pubmed-10276549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102765492023-06-18 Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows Almeida, Kleves V Resende, Tales L Silva, Luiz Henrique P Dorich, Christopher D Pereira, Andre B D Soder, Kathy J Brito, Andre F Transl Anim Sci Ruminant Nutrition We evaluated the effects of incremental amounts of ground flaxseed (GFX) on diversity and relative abundance of ruminal microbiota taxa, enteric methane (CH(4)) emissions, and urinary excretion of purine derivatives (PD) in lactating dairy cows in a replicated 4 × 4 Latin square design. Twenty mid-lactation Jersey cows were used in the study. Of these 20 cows, 12 were used for ruminal sampling, 16 for enteric CH(4) measurements, and all for spot urine collection. Each period lasted 21 d with 14 d for diet adaptation and 7 d for data and sample collection. Diets were formulated by replacing corn meal and soybean meal with 0%, 5%, 10%, and 15% of GFX in the diet’s dry matter. Ruminal fluid samples obtained via stomach tubing were used for DNA extraction. Enteric CH(4) production was measured using the sulfur hexafluoride tracer technique. Diets had no effect on ruminal microbiota diversity. Similarly, the relative abundance of ruminal archaea genera was not affected by diets. In contrast, GFX decreased or increased linearly the relative abundance of Firmicutes (P < 0.01) and Bacteroidetes (P < 0.01), respectively. The relative abundance of the ruminal bacteria Ruminococcus (P < 0.01) and Clostridium (P < 0.01) decreased linearly, and that of Prevotella (P < 0.01) and Pseudobutyrivibrio (P < 0.01) increased linearly with feeding GFX. A tendency for a linear reduction (P = 0.055) in enteric CH(4) production (from 304 to 256 g/d) was observed in cows fed increasing amounts of GFX. However, neither CH(4) yield nor CH(4) intensity was affected by treatments. Diets had no effect on the urinary excretion of uric acid, allantoin, and total PD. Overall, feeding GFX decreased linearly the relative abundance of the ruminal bacterial genera Ruminococcus and Clostridium and enteric CH(4) production, but no change was seen for CH(4) yield and CH(4) intensity, or urinary excretion of total PD, suggesting no detrimental effect of GFX on microbial protein synthesis in the rumen. Oxford University Press 2023-05-29 /pmc/articles/PMC10276549/ /pubmed/37334244 http://dx.doi.org/10.1093/tas/txad050 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Ruminant Nutrition Almeida, Kleves V Resende, Tales L Silva, Luiz Henrique P Dorich, Christopher D Pereira, Andre B D Soder, Kathy J Brito, Andre F Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title | Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title_full | Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title_fullStr | Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title_full_unstemmed | Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title_short | Feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
title_sort | feeding incremental amounts of ground flaxseed: effects on diversity and relative abundance of ruminal microbiota and enteric methane emissions in lactating dairy cows |
topic | Ruminant Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10276549/ https://www.ncbi.nlm.nih.gov/pubmed/37334244 http://dx.doi.org/10.1093/tas/txad050 |
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