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Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation
Since its first appearance in late 2019 in Wuhan, China, severe acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2) has had a major impact on healthcare facilities around the world. Although in the past year, mass vaccination and the development of monoclonal antibody treatments have red...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277158/ https://www.ncbi.nlm.nih.gov/pubmed/37366528 http://dx.doi.org/10.1016/j.heliyon.2023.e17438 |
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author | Scaglione, Sara Gotta, Franca Vay, Daria Leli, Christian Roveta, Annalisa Maconi, Antonio Rocchetti, Andrea |
author_facet | Scaglione, Sara Gotta, Franca Vay, Daria Leli, Christian Roveta, Annalisa Maconi, Antonio Rocchetti, Andrea |
author_sort | Scaglione, Sara |
collection | PubMed |
description | Since its first appearance in late 2019 in Wuhan, China, severe acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2) has had a major impact on healthcare facilities around the world. Although in the past year, mass vaccination and the development of monoclonal antibody treatments have reduced the number of deaths and severe cases, the circulation of SARS-CoV-2 remains high. Over the past two years, diagnostics have played a crucial role in virus containment both in health care facilities and at the community level. For SARS-CoV-2 detection, the commonly used specimen type is the nasopharyngeal swab, although the virus can be identified in other matrices such as feces. Since fecal microbiota transplantation (FMT) assumes significant importance in the treatment of chronic gut infections and that feces may be a potential vehicle for transmission of SARS-CoV-2, in this study we have evaluated the performance of the rapid cartridge-based RT-PCR test STANDARD™ M10 SARS-CoV-2 (SD Biosensor Inc., Suwon, South Korea) using fecal samples. The results obtained indicates that STANDARD™ M10 SARS-CoV-2 can detect SARS-CoV-2 in stool samples even at low concentration. For this reason, STANDARD™ M10 SARS-CoV-2 could be used as reliable methods for the detection of SARS-CoV-2 in fecal samples and for the screening of FMT donors. |
format | Online Article Text |
id | pubmed-10277158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-102771582023-06-21 Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation Scaglione, Sara Gotta, Franca Vay, Daria Leli, Christian Roveta, Annalisa Maconi, Antonio Rocchetti, Andrea Heliyon Research Article Since its first appearance in late 2019 in Wuhan, China, severe acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2) has had a major impact on healthcare facilities around the world. Although in the past year, mass vaccination and the development of monoclonal antibody treatments have reduced the number of deaths and severe cases, the circulation of SARS-CoV-2 remains high. Over the past two years, diagnostics have played a crucial role in virus containment both in health care facilities and at the community level. For SARS-CoV-2 detection, the commonly used specimen type is the nasopharyngeal swab, although the virus can be identified in other matrices such as feces. Since fecal microbiota transplantation (FMT) assumes significant importance in the treatment of chronic gut infections and that feces may be a potential vehicle for transmission of SARS-CoV-2, in this study we have evaluated the performance of the rapid cartridge-based RT-PCR test STANDARD™ M10 SARS-CoV-2 (SD Biosensor Inc., Suwon, South Korea) using fecal samples. The results obtained indicates that STANDARD™ M10 SARS-CoV-2 can detect SARS-CoV-2 in stool samples even at low concentration. For this reason, STANDARD™ M10 SARS-CoV-2 could be used as reliable methods for the detection of SARS-CoV-2 in fecal samples and for the screening of FMT donors. Elsevier 2023-06-18 /pmc/articles/PMC10277158/ /pubmed/37366528 http://dx.doi.org/10.1016/j.heliyon.2023.e17438 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Scaglione, Sara Gotta, Franca Vay, Daria Leli, Christian Roveta, Annalisa Maconi, Antonio Rocchetti, Andrea Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title | Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title_full | Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title_fullStr | Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title_full_unstemmed | Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title_short | Rapid RT-PCR identification of SARS-CoV-2 in screening donors of fecal microbiota transplantation |
title_sort | rapid rt-pcr identification of sars-cov-2 in screening donors of fecal microbiota transplantation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277158/ https://www.ncbi.nlm.nih.gov/pubmed/37366528 http://dx.doi.org/10.1016/j.heliyon.2023.e17438 |
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