Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines

Combination therapy using effective drug molecules and functional genes such as small interfering RNA (siRNA) has been suggested as a powerful strategy against multiple drug resistance. Here, we present a protocol for preparing a delivery system by developing dynamic covalent macrocycles using a dit...

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Detalles Bibliográficos
Autores principales: Lyu, Yonglei, Wu, Xiaoxia, Yang, Jinghui, Wang, Xin, Li, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277607/
https://www.ncbi.nlm.nih.gov/pubmed/37314921
http://dx.doi.org/10.1016/j.xpro.2023.102350
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author Lyu, Yonglei
Wu, Xiaoxia
Yang, Jinghui
Wang, Xin
Li, Jianwei
author_facet Lyu, Yonglei
Wu, Xiaoxia
Yang, Jinghui
Wang, Xin
Li, Jianwei
author_sort Lyu, Yonglei
collection PubMed
description Combination therapy using effective drug molecules and functional genes such as small interfering RNA (siRNA) has been suggested as a powerful strategy against multiple drug resistance. Here, we present a protocol for preparing a delivery system by developing dynamic covalent macrocycles using a dithiol monomer to co-deliver doxorubicin and siRNA. We describe steps for preparing the dithiol monomer, followed by co-delivery to form nanoparticles. We then detail procedures for cell uptake and assessing enhanced anti-cancer efficacy in vitro. For complete details on the use and execution of this protocol, please refer to Lyu et al.(1)
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spelling pubmed-102776072023-06-20 Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines Lyu, Yonglei Wu, Xiaoxia Yang, Jinghui Wang, Xin Li, Jianwei STAR Protoc Protocol Combination therapy using effective drug molecules and functional genes such as small interfering RNA (siRNA) has been suggested as a powerful strategy against multiple drug resistance. Here, we present a protocol for preparing a delivery system by developing dynamic covalent macrocycles using a dithiol monomer to co-deliver doxorubicin and siRNA. We describe steps for preparing the dithiol monomer, followed by co-delivery to form nanoparticles. We then detail procedures for cell uptake and assessing enhanced anti-cancer efficacy in vitro. For complete details on the use and execution of this protocol, please refer to Lyu et al.(1) Elsevier 2023-06-13 /pmc/articles/PMC10277607/ /pubmed/37314921 http://dx.doi.org/10.1016/j.xpro.2023.102350 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lyu, Yonglei
Wu, Xiaoxia
Yang, Jinghui
Wang, Xin
Li, Jianwei
Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title_full Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title_fullStr Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title_full_unstemmed Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title_short Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
title_sort protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277607/
https://www.ncbi.nlm.nih.gov/pubmed/37314921
http://dx.doi.org/10.1016/j.xpro.2023.102350
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